Linked Life Data

6644188

Source:http://linkedlifedata.com/resource/pubmed/id/6644188

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1984-1-27
pubmed:abstractText
A reversed-phase high performance liquid chromatography (HPLC) technique was developed for the analysis of sphingoid bases as their biphenylcarbonyl derivatives. The sphingoid bases (0.3-500 nmol) obtained from sphingolipids after hydrolysis were quantitatively derivatized with biphenylcarbonyl chloride at room temperature for 90 min. After removal of excess reagent by partitioning, the derivatized products were quantitatively analyzed on a reversed-phase HPLC column, with detection at 280 nm. A mixture of biphenylcarbonyl C18-5-hydroxysphinganine, C18-erythro and threo sphingenine, and sphinganine, C18-5-O-methyl and 3-O-methyl sphingenine, C20-sphingenine, sphingosyl phosphorylcholine, and psychosine and were well resolved from one another. The method was employed for the analysis of less than nanomole quantities of sphingoid bases from sphingomyelin and cerebrosides.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-2275
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1380-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Analysis of sphingoid bases by reversed-phase high performance liquid chromatography.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.