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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1984-1-7
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pubmed:abstractText |
An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of rabbit immunoglobulin G (IgG) antibodies to purified cell wall teichoic acids from the Staphylococcus aureus Lafferty strain and three strains of coagulase-negative staphylococci. Significant immunological cross-reactivity occurred only between the teichoic acid of S. aureus and one coagulase-negative preparation. The ELISA was used to determine the serum IgG response to Staphylococcus epidermidis in a rabbit model of aortic valve endocarditis. Blood samples were drawn before inoculation and then every 5 days until death or sacrifice at 32 to 35 days postinoculation. Valve vegetations were culture positive at autopsy in 16 (59%) of the 27 catheterized rabbits. Antibody titers in this culture-positive group and the culture-negative group began to rise as early as day 6. Although both groups demonstrated an antibody response, the culture-positive group attained a significantly higher titer on days 26 and 31. Antibodies also rose in a control group of rabbits without a heart catheter but which were inoculated with bacteria. Again, the antibody titer was significantly less than that for the culture-positive group. This ELISA may be useful for the diagnosis of coagulase-negative staphylococcal infections in humans.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6642658-1127260,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6642658-1171577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6642658-13469408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6642658-13570722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6642658-14048670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6642658-14048671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6642658-14190074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6642658-193356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6642658-4113792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6642658-415094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6642658-4626387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6642658-46620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6642658-50716,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6642658-5123055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6642658-518218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6642658-641141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6642658-7053681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6642658-7264368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6642658-79624,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6642658-829022
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
42
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1020-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6642658-Animals,
pubmed-meshheading:6642658-Antibodies, Bacterial,
pubmed-meshheading:6642658-Cell Wall,
pubmed-meshheading:6642658-Cross Reactions,
pubmed-meshheading:6642658-Endocarditis, Bacterial,
pubmed-meshheading:6642658-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:6642658-Immunoglobulin G,
pubmed-meshheading:6642658-Rabbits,
pubmed-meshheading:6642658-Staphylococcal Infections,
pubmed-meshheading:6642658-Staphylococcus epidermidis,
pubmed-meshheading:6642658-Teichoic Acids
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pubmed:year |
1983
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pubmed:articleTitle |
Detection of anti-teichoic acid immunoglobulin G antibodies in experimental Staphylococcus epidermidis endocarditis.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, Non-P.H.S.
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