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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
|
| pubmed:dateCreated |
1983-12-20
|
| pubmed:abstractText |
The capacity of embryonic chick myoblasts to grow in vitro on DEAE-cellulose microcarriers (MC) has been investigated biochemically and morphologically. The cells attached to the MC, replicated and fused to form elongated myotubes. These myotubes synthesized muscle-specific proteins, such as creatine kinase (CK) and acetylcholine receptors (AChR), and they contracted spontaneously. Some of the advantages of this technique are: a) Tridimensional development of myotubes on MC with orientation of fibers parallel to each other; b) Muscle cells can be cultured on MC for long periods (months); c) Easy harvesting of samples at any time during cultivation; d) DEAE-cellulose MC are commercially available, inexpensive and easy to handle.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0309-1651
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
7
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
727-34
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:6627408-Animals,
pubmed-meshheading:6627408-Cell Differentiation,
pubmed-meshheading:6627408-Cell Division,
pubmed-meshheading:6627408-Cells, Cultured,
pubmed-meshheading:6627408-Chick Embryo,
pubmed-meshheading:6627408-Microscopy, Electron,
pubmed-meshheading:6627408-Microscopy, Electron, Scanning,
pubmed-meshheading:6627408-Muscles
|
| pubmed:year |
1983
|
| pubmed:articleTitle |
Myogenesis on microcarrier cultures.
|
| pubmed:publicationType |
Journal Article
|