Linked Life Data

6626556

Source:http://linkedlifedata.com/resource/pubmed/id/6626556

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1983-12-17
pubmed:abstractText
The procoagulant component has been purified from timber rattlesnake (Crotalus horridus horridus) venom by DEAE-cellulose ion-exchange chromatography followed by affinity chromatography on immobilized p-aminobenzamidine and a final DEAE-Sepharose chromatography. As obtained, the procoagulant gave a single band of Mr 29 500 +/- 2000 on SDS-polyacrylamide gel electrophoresis whether or not the sample was reduced prior to electrophoresis. Schiff's stain indicated the presence of some carbohydrate. The procoagulant showed one predominant and four minor bands on discontinuous gel electrophoresis. All caused fibrinogen solutions to clot. Treatment of the preparation with neuraminidase did not cause the minor bands to comigrate with the major band. Amino acid analysis revealed the presence of eight half-cystines, all of which were present as cystines in the intact molecule. The procoagulant has 13 tryptophans per molecule and an extinction coefficient for a 1% solution at 280 nm of 26.3. This venom procoagulant was found to induce clotting by catalyzing the hydrolysis of only the A fibrinopeptide from the A alpha-chain of fibrinogen. It was not inhibited by protein trypsin inhibitors, N-ethylmaleimide or dithiothreitol, but it was inactivated by phenylmethylsulfonyl fluoride, indicating an active-center serine. The procoagulant catalyzed the release of negligible acid-soluble peptides from bovine serum albumin, casein and hemoglobin.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
748
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
236-44
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
A thrombin-like enzyme from timber rattlesnake venom.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.