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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
1983-11-23
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pubmed:abstractText |
Assay procedure were developed for a soluble glycerol kinase (apparent Km (glycerol) 9 microM) and a probably membrane-associated, NAD-independent L-glycerol-3-phosphate dehydrogenase [apparent Km (L-glycerol 3-phosphate) 7 mM] present in Streptomyces coelicolor A3(2). Both enzymes were cold sensitive. They were co-ordinately induced (about 35-fold) by addition of glycerol to cultures growing on arabinose as sole carbon source. Induction was rifampicin sensitive. The dehydrogenase was absent from glycerol-sensitive mutants, and both kinase and dehydrogenase were absent from glycerol non-utilizing (but glycerol-resistant) mutants, demonstrating that the two enzymes are part of the major pathway of glycerol catabolism in S. coelicolor. Circumstantial evidence suggested that their inducer is glycerol 3-phosphate rather than glycerol. The enzymes were subject to co-ordinate repression by various carbon sources, of which glucose exerted the strongest effect (a fivefold repression). Previously described mutants resistant to 2-deoxyglucose, shown here to have very low glucose kinase activity, were defective in glucose repression of the glycerol enzymes.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Glycerol,
http://linkedlifedata.com/resource/pubmed/chemical/Glycerol Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Glycerol-3-Phosphate Dehydrogenase...,
http://linkedlifedata.com/resource/pubmed/chemical/Glycerolphosphate Dehydrogenase
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-1287
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
129
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1403-13
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:6619799-Enzyme Induction,
pubmed-meshheading:6619799-Gene Expression Regulation,
pubmed-meshheading:6619799-Glucose,
pubmed-meshheading:6619799-Glycerol,
pubmed-meshheading:6619799-Glycerol Kinase,
pubmed-meshheading:6619799-Glycerol-3-Phosphate Dehydrogenase (NAD+),
pubmed-meshheading:6619799-Glycerolphosphate Dehydrogenase,
pubmed-meshheading:6619799-Mutation,
pubmed-meshheading:6619799-Streptomyces
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pubmed:year |
1983
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pubmed:articleTitle |
Glycerol catabolic enzymes and their regulation in wild-type and mutant strains of Streptomyces coelicolor A3(2).
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pubmed:publicationType |
Journal Article
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