Linked Life Data

6619572

Source:http://linkedlifedata.com/resource/pubmed/id/6619572

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1983-11-23
pubmed:abstractText
Intracellular parasitism of host macrophages is the pathologic hallmark of leishmaniasis. Since the organisms are found almost exclusively in this type of cell, the possibility that specific macrophage plasma-membrane determinants mediate the attachment to promastigotes of Leishmania tropica was investigated. Plasma membrane vesicles were prepared from human monocytes/macrophages and erythrocytes, radioiodinated, and incubated with L tropica promastigotes, erythrocytes, or Sephadex beads. Macrophage plasma-membrane vesicles bound to promastigotes to a significant extent but did not bind to intact erythrocytes or to an inert particle. In contrast, erythrocyte membrane vesicles did not bind to promastigotes. The binding of macrophage plasma-membrane vesicles to promastigotes demonstrated the characteristics of a receptor-ligand interaction in terms of specificity, saturability, competitive inhibition, and temperature independence. These results suggest the presence of one or more intrinsic binding sites on the macrophage plasma membrane to which promastigotes can attach. If this is the case, therapeutic intervention by strategies that inhibit attachment of this obligate intracellular parasite to its target host cell may be possible.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-1899
pubmed:author
pubmed:issnType
Print
pubmed:volume
148
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
377-84
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Attachment of plasma membrane vesicles of human macrophages to Leishmania tropica promastigotes.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't