Linked Life Data

6610177

Source:http://linkedlifedata.com/resource/pubmed/id/6610177

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1984-7-24
pubmed:abstractText
The cytotoxic reactivity of lymphocytes for autologous melanoma cells was studied in a group of 13 melanoma patients. No cytotoxicity was observed with lymphocytes freshly isolated from peripheral blood or with lymphocytes cocultured for 7 days with autologous melanoma cells. Growth of lymphocytes previously sensitized with autologous melanoma in vitro in interleukin 2 (IL-2)-containing medium, however, resulted in cytotoxic reactivity for autologous melanoma in 7/13 patients. The reactivity of IL-2-dependent lymphocytes for autologous melanoma was particularly striking in one patient (A.V.) who has had an unexpectedly favorable clinical course and, because of their consistently high reactivity, AV lymphocytes were selected for detailed specificity analysis. After 2-3 weeks in culture in IL-2-containing medium, AV lymphocytes were cytolytic for autologous melanoma cells but not autologous Epstein-Barr virus-transformed B cells, autologous fibroblasts, or allogeneic tumor targets. Specificity of autologous melanoma reactivity was confirmed by competitive inhibition assays. The IL-2-dependent AV lymphocytes formed rosettes with sheep erythrocytes and expressed OKT 3 and Ia antigens. After longer periods of culture, AV lymphocytes were found to react with a wider range of target cells, and repeated attempts to isolate cultures with restricted reactivity to autologous melanoma by resensitization with autologous melanoma and limiting-dilution techniques were unsuccessful. The restricted reactivity of early cultures could be preserved, however, in frozen storage, but shifted again toward broader reactivity after several weeks in culture. The recognition of cytotoxic T cells with initial restricted reactivity for autologous melanoma suggests reinvestigation of the question of specific cellular immunity to human cancer.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6610177-1067619, http://linkedlifedata.com/resource/pubmed/commentcorrection/6610177-13502695, http://linkedlifedata.com/resource/pubmed/commentcorrection/6610177-145543, http://linkedlifedata.com/resource/pubmed/commentcorrection/6610177-181845, http://linkedlifedata.com/resource/pubmed/commentcorrection/6610177-270702, http://linkedlifedata.com/resource/pubmed/commentcorrection/6610177-283420, http://linkedlifedata.com/resource/pubmed/commentcorrection/6610177-478690, http://linkedlifedata.com/resource/pubmed/commentcorrection/6610177-479762, http://linkedlifedata.com/resource/pubmed/commentcorrection/6610177-6602860, http://linkedlifedata.com/resource/pubmed/commentcorrection/6610177-6976407, http://linkedlifedata.com/resource/pubmed/commentcorrection/6610177-7004632, http://linkedlifedata.com/resource/pubmed/commentcorrection/6610177-7141736, http://linkedlifedata.com/resource/pubmed/commentcorrection/6610177-978138
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
81
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3511-5
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
T-cell-mediated cytotoxicity against autologous malignant melanoma: analysis with interleukin 2-dependent T-cell cultures.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't