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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
1984-6-27
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pubmed:abstractText |
HLA-A2 antigen mutants were obtained previously from the B lymphoblastoid cell line T5-1 by mutagenesis followed by immunoselection. Here we present biochemical studies of one particular mutant, clone 8.14.1. These cells synthesize two forms of HLA-A2: a minor form, which remains cell-associated at all times, and an abundant form, which is secreted. The former appears by SDS-PAGE to be slightly larger than T5-1 HLA-A2, whereas the latter appears to be 4000 to 5000 daltons smaller. In vitro translation and in vivo pulse-chase studies suggest that these species are not related to each other by post-translational processing. Proteolytic digestion studies localize the resulting structural alteration in the mobility difference between wild-type and secreted HLA-A2 to a region near the carboxy terminus of the HLA-A2 heavy chain; however, their extreme carboxy termini appear similar, if not identical. We suggest that the secreted form may result from a pattern of RNA splicing in which the exon encoding the hydrophobic, membrane-spanning region is frequently deleted.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-1767
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
132
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2984-91
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:6609965-Antibodies, Monoclonal,
pubmed-meshheading:6609965-Antigen-Antibody Reactions,
pubmed-meshheading:6609965-B-Lymphocytes,
pubmed-meshheading:6609965-Cell Line,
pubmed-meshheading:6609965-Chemical Precipitation,
pubmed-meshheading:6609965-Chromatography, Gel,
pubmed-meshheading:6609965-Clone Cells,
pubmed-meshheading:6609965-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:6609965-HLA Antigens,
pubmed-meshheading:6609965-HLA-A2 Antigen,
pubmed-meshheading:6609965-Humans,
pubmed-meshheading:6609965-Mutation,
pubmed-meshheading:6609965-Protein Biosynthesis
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pubmed:year |
1984
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pubmed:articleTitle |
Characterization of a B lymphoblastoid cell line mutant that secretes HLA-A2.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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