Linked Life Data

6607844

Source:http://linkedlifedata.com/resource/pubmed/id/6607844

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1984-4-20
pubmed:abstractText
Some biological and biochemical properties of a distinct hemopoietic factor that stimulates splenic hemopoiesis in mice are described. This factor can be detected by measuring the increase in the number of in vitro hemopoietic colony-forming cells (CFCs) in the spleens of mice after transfer of serum from syngeneic donors that have been treated previously with the bacterial cell-wall components: lipid A or lipoprotein. Serum collected 5 min after the IV injection of lipid A contained almost no splenic hemopoiesis-stimulating factor (SHSF). The highest serum levels of the factor were found between 30 min and 3 h after lipid A was injected IV. The residual levels of lipid A or lipoprotein in the serum of treated mice were too low to account for their splenic hemopoiesis-stimulating effects. A component of SHSF in both post-lipid-A serum (PLAS) and postlipoprotein serum (PLPS) bound to concanavalin A (Con A)-Sepharose and could be eluted by alpha-methyglucopyranoside (0.05 M). Partial fractionation of PLAS using Con A-Sepharose and gel filtration (Sephacryl S-200) indicated that the SHSF glycoprotein had an apparent molecular weight of 30,000 daltons. SHSF was detected in serum in response to lipid A and lipoprotein, but this was separable (by gel filtration) from the major form of granulocyte-macrophage colony-stimulating factor (GM-CSF) in PLAS.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0301-472X
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
107-13
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Biological and biochemical properties of a serum factor that stimulates splenic hemopoiesis in mice.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't