Linked Life Data

6605386

Source:http://linkedlifedata.com/resource/pubmed/id/6605386

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1983-12-17
pubmed:abstractText
Contrasting effects of interferon alpha + beta (IFN) on colony-stimulating factor- (CSF) induced macrophage stem cell proliferation resulted when nucleated murine femoral marrow cells were exposed to IFN either before addition of CSF or simultaneously with CSF. Responsiveness to macrophage type L cell CSF was measured by two different assays: by determination of [3H]thymidine (3H-TdR) incorporation and by enumeration of colonies formed in response to CSF. Incubation of nucleated marrow cells concurrently with CSF and IFN resulted in depressed proliferation that was dependent on the dose of IFN. Preincubation of marrow cells with IFN, however, produced a dose-dependent enhancement of CSF responsiveness in comparison to preincubation of marrow cells in the absence of IFN. The enhancing effect of IFN was observed only when preincubated cells were stimulated with suboptimal concentrations of CSF. At optimal CSF concentrations, differences were not observed in either 3H-TdR incorporation or in colony formation. On the basis of data accumulated by both assays for CSF responsiveness, IFN pretreatment was hypothesized not to increase the total population of existing progenitor cells, but to influence the capacity of pre-existing progenitor cells to respond to suboptimal doses of the growth factor.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:volume
131
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2374-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Enhanced responsiveness of committed macrophage precursors to macrophage-type colony-stimulating factor (CSF-1) induced in vitro by interferons alpha + beta 1.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.