Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1983-10-8
pubmed:abstractText
Our previous studies indicated that cloned T cells obtained from Peyer's patches (PP) (Lyt-1+, 2-, Ia+, and H-2K/D+) evoked immunoglobulin (Ig) class switching of PP B cells from sIgM to sIgA cells in vitro; however, these switch T cells could not in themselves provide optimal help for the differentiation of postswitch sIgA-bearing PP B cells to IgA-secreting cells. Thus, in the present report we described studies focused on mechanisms regulating terminal differentiation of the postswitch PP sIgA-bearing B cells. First, to explore the effect of T cell-derived B cell differentiation factor(s) (BCDF) and macrophage factor(s) (MF) on the terminal maturation of PP B cells, LPS-stimulated PP B cells were co-cultured for 7 d with cloned T cells in the presence or absence of the above factors. In the absence of PP cloned T cells the BCDF and MF had only a modest effect on IgA production, whereas in the presence of PP, but not spleen cloned T cells, IgA production was increased. Next, to investigate the effect of T cells derived from a gut-associated lymphoid tissue (GALT), mesenteric lymph nodes (MLN), as well as from spleen on terminal differentiation of postswitch sIgA PP B cells, LPS-driven PP B cells were precultured with the cloned T cells to induce a switch to sIgA, and subsequently cultured with MLN or spleen T cells or a Lyt-2+-depleted T cell subset in the presence of a T-dependent polyclonal mitogen, staphylococcal protein A. Alternatively, in the second culture period BCDF alone was added, instead of T cells and protein A. Here it was found that B cells pre-exposed to switch T cells from PP, but not spleen, were induced to produce greatly increased amounts of IgA in the presence of protein A and T cells or a Lyt-2+-depleted T cell subset as well as in the presence of BCDF alone. Furthermore, in the presence of BCDF alone many B cells expressed cytoplasmic IgA. These observations strongly support the view that the terminal differentiation of postswitch sIgA B cells is governed by helper T cells and macrophages, or factors derived from such cells. Such cells or factors do not affect preswitch B cells.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6604126-1080773, http://linkedlifedata.com/resource/pubmed/commentcorrection/6604126-109511, http://linkedlifedata.com/resource/pubmed/commentcorrection/6604126-115808, http://linkedlifedata.com/resource/pubmed/commentcorrection/6604126-149174, http://linkedlifedata.com/resource/pubmed/commentcorrection/6604126-305448, http://linkedlifedata.com/resource/pubmed/commentcorrection/6604126-307029, http://linkedlifedata.com/resource/pubmed/commentcorrection/6604126-311811, http://linkedlifedata.com/resource/pubmed/commentcorrection/6604126-361892, http://linkedlifedata.com/resource/pubmed/commentcorrection/6604126-394643, http://linkedlifedata.com/resource/pubmed/commentcorrection/6604126-394644, http://linkedlifedata.com/resource/pubmed/commentcorrection/6604126-4919144, http://linkedlifedata.com/resource/pubmed/commentcorrection/6604126-4934147, http://linkedlifedata.com/resource/pubmed/commentcorrection/6604126-563294, http://linkedlifedata.com/resource/pubmed/commentcorrection/6604126-6164719, http://linkedlifedata.com/resource/pubmed/commentcorrection/6604126-6185611, http://linkedlifedata.com/resource/pubmed/commentcorrection/6604126-620430, http://linkedlifedata.com/resource/pubmed/commentcorrection/6604126-6776537, http://linkedlifedata.com/resource/pubmed/commentcorrection/6604126-6787609, http://linkedlifedata.com/resource/pubmed/commentcorrection/6604126-6795304, http://linkedlifedata.com/resource/pubmed/commentcorrection/6604126-6804369, http://linkedlifedata.com/resource/pubmed/commentcorrection/6604126-6806372, http://linkedlifedata.com/resource/pubmed/commentcorrection/6604126-6965298, http://linkedlifedata.com/resource/pubmed/commentcorrection/6604126-6968339, http://linkedlifedata.com/resource/pubmed/commentcorrection/6604126-6975305, http://linkedlifedata.com/resource/pubmed/commentcorrection/6604126-7000673, http://linkedlifedata.com/resource/pubmed/commentcorrection/6604126-7021394, http://linkedlifedata.com/resource/pubmed/commentcorrection/6604126-722238, http://linkedlifedata.com/resource/pubmed/commentcorrection/6604126-88758
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-1007
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
158
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
649-69
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:6604126-Animals, pubmed-meshheading:6604126-Antibody Specificity, pubmed-meshheading:6604126-Antibody-Producing Cells, pubmed-meshheading:6604126-B-Lymphocytes, pubmed-meshheading:6604126-Clone Cells, pubmed-meshheading:6604126-Female, pubmed-meshheading:6604126-Growth Substances, pubmed-meshheading:6604126-Immunoglobulin A, pubmed-meshheading:6604126-Immunoglobulin G, pubmed-meshheading:6604126-Immunoglobulin M, pubmed-meshheading:6604126-Interleukin-2, pubmed-meshheading:6604126-Interleukin-4, pubmed-meshheading:6604126-Lipopolysaccharides, pubmed-meshheading:6604126-Lymph Nodes, pubmed-meshheading:6604126-Lymphoid Tissue, pubmed-meshheading:6604126-Macrophages, pubmed-meshheading:6604126-Mice, pubmed-meshheading:6604126-Mice, Inbred BALB C, pubmed-meshheading:6604126-Peyer's Patches, pubmed-meshheading:6604126-Rats, pubmed-meshheading:6604126-Rats, Inbred Strains, pubmed-meshheading:6604126-T-Lymphocytes
pubmed:year
1983
pubmed:articleTitle
Mechanisms regulating IgA class-specific immunoglobulin production in murine gut-associated lymphoid tissues. II. Terminal differentiation of postswitch sIgA-bearing Peyer's patch B cells.
pubmed:publicationType
Journal Article