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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1983-10-28
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pubmed:abstractText |
Cell swelling and elevated intracellular Ca2+ increase K+ permeability in lymphocytes. Experiments were performed to test whether these effects can also be elicited in isolated plasma membrane vesicles. Rabbit thymocytes, used as a source of membrane vesicles, were found to regain their volume after swelling in hypotonic, low-K+ media. This regulatory volume decrease (RVD) was inhibited by quinine and trifluoperazine, but not affected by ouabain. Both efflux and uptake of K+ (86Rb) were stimulated by hypotonicity. Addition of A23187 plus Ca2+ also increased 86Rb fluxes. Ca2+- and volume-induced 86Rb fluxes were also studied in isolated membranes. A plasma membrane-rich vesicle fraction, enriched over 11-fold in 5'-nucleotidase, was isolated from thymocytes. The vesicles were about 35% inside-out and trapped 86Rb in an osmotically active compartment of approximately 1.3 microliter/mg protein. Equilibrium exchange fluxes of 86Rb in the vesicles were unaffected by Ca2+ with or without A23187. Calmodulin had no effect on 86Rb permeability but stimulated ATP-dependent Ca2+ accumulation. Hypotonic swelling increased both uptake and efflux of 86Rb from vesicles. However, this increase was not blocked by either quinine or trifluoperazine, was not specific for K+ (86Rb), and is probably unrelated to RVD. It is concluded that components essential for the volume- and Ca2+-induced changes in K+ permeability are lost or inactivated during membrane isolation. An intact cytoarchitecture may be required for RVD.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calmodulin,
http://linkedlifedata.com/resource/pubmed/chemical/Hypotonic Solutions,
http://linkedlifedata.com/resource/pubmed/chemical/Ouabain,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium,
http://linkedlifedata.com/resource/pubmed/chemical/Quinine,
http://linkedlifedata.com/resource/pubmed/chemical/Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Rubidium,
http://linkedlifedata.com/resource/pubmed/chemical/Trifluoperazine
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0021-9541
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
116
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
352-62
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:6604061-Animals,
pubmed-meshheading:6604061-Calcium,
pubmed-meshheading:6604061-Calmodulin,
pubmed-meshheading:6604061-Cell Membrane,
pubmed-meshheading:6604061-Cell Membrane Permeability,
pubmed-meshheading:6604061-Hypotonic Solutions,
pubmed-meshheading:6604061-Male,
pubmed-meshheading:6604061-Ouabain,
pubmed-meshheading:6604061-Potassium,
pubmed-meshheading:6604061-Quinine,
pubmed-meshheading:6604061-Rabbits,
pubmed-meshheading:6604061-Radioisotopes,
pubmed-meshheading:6604061-Rubidium,
pubmed-meshheading:6604061-T-Lymphocytes,
pubmed-meshheading:6604061-Trifluoperazine
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pubmed:year |
1983
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pubmed:articleTitle |
Induction of 86Rb fluxes by Ca2+ and volume changes in thymocytes and their isolated membranes.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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