Linked Life Data

6602888

Source:http://linkedlifedata.com/resource/pubmed/id/6602888

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1983-8-11
pubmed:abstractText
Different populations of human effector cells were examined for their ability to bind and to lyse K562 target cells as monitored by a fluorochrome-labeled batch system binding assay and a 6-hour chromium release assay respectively. Binding and cytolysis were found to increase, or decrease, concomitantly with null cell purification, and both were abrogated by trypsin treatment of effectors. At 4 degrees C, cytolysis was abolished whereas binding was only decreased. When binding and cytolysis data were correlated, the nylon wool nonadherent cells (i.e., T and null cells) were found to be the most efficient (i.e., fewer bound effectors per target cell lysed) while mononuclear cells were the least efficient with respect to natural killing. These findings support the current hypotheses of spontaneous killer ontogeny and peripheral blood compartmentalization. Furthermore, this study confirms observations made for mononuclear and enriched T plus null cell populations in single conjugate in agarose assays and extends those to the null cell population--a population relatively enriched for large granular lymphocytes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0141-2760
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
203-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Human natural killer cells: correlation of a lytic assay with a visual binding assay.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.