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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1983-5-5
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pubmed:abstractText |
A protein identified as P85(gag-mos) was shown to be phosphorylated when immunoprecipitates from ts110 Moloney murine sarcoma virus transformed nonproducer cells (clone 6m2) were incubated with [gamma-(32)P]ATP. The in vitro-labeled 85,000-dalton phosphoprotein comigrated on NaDodSO(4)/polyacrylamide gels with authentic phosphorylated P85(gag-mos). Immunoprecipitates obtained with antisera prepared against Rauscher murine leukemia virus core protein p30 were active in the immune complex kinase assay but anti-murine leukemia virus p10 precipitates were not. Previous studies have shown that anti-p30 but not anti-p10 antisera recognize P85(gag-mos). The 6m2 clone has been shown to express P85(gag-mos) at 33 degrees C but not at 39 degrees C. Anti-p30 immune complexes from 6m2 cells maintained at 39 degrees C failed to phosphorylate the 85,000-dalton protein. Furthermore, the in vitro phosphorylated 85,000-dalton protein gave the same pattern of V8 protease-generated cleavage products as in vivo(32)P-labeled P85(gag-mos). We conclude from these results that P85(gag-mos) is phosphorylated in anti-p30 immune complex kinase reactions. Phosphoamino acid analyses indicated that the in vitro phosphorylated P85(gag-mos) contained phosphoserine and phosphothreonine. Our findings indicate that incubation of anti-p30 immunoprecipitates at 39 degrees C drastically reduced, in a specific way, the kinase activity associated with P85(gag-mos). This result and other data suggest that the kinase is virus-encoded. Because P85(gag-mos), but not Pr65(gag) is phosphorylated in anti-p30 immunoprecipitates from MuLV-MuSV ts110 producer cells, the kinase enzyme is associated with P85(gag-mos) and not gag gene products. A second major polypeptide of the size of P58(gag) was also phosphorylated in anti-p30 immunoprecipitates from cells maintained at 33 degrees C but not at 39 degrees C. Since 6m2 cells at 39 degrees C contain P58(gag), this is also consistent with the kinase activity being associated with P85(gag-mos).
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6601272-1102604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6601272-205879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6601272-214242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6601272-226971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6601272-320200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6601272-4318987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6601272-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6601272-6100962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6601272-6168778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6601272-6170110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6601272-6173134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6601272-6173767,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6601272-6243819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6601272-6244493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6601272-6251974,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6601272-6253810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6601272-6254060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6601272-6255480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6601272-6256659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6601272-6257397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6601272-6283546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6601272-6285367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6601272-6288258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6601272-67705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6601272-6946495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6601272-6955449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6601272-6981878,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6601272-7273137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6601272-7423857
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
80
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
412-6
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:6601272-Animals,
pubmed-meshheading:6601272-Antigen-Antibody Complex,
pubmed-meshheading:6601272-Cell Line,
pubmed-meshheading:6601272-Gene Products, gag,
pubmed-meshheading:6601272-Genes,
pubmed-meshheading:6601272-Genes, Viral,
pubmed-meshheading:6601272-Immune Sera,
pubmed-meshheading:6601272-Kidney,
pubmed-meshheading:6601272-Kinetics,
pubmed-meshheading:6601272-Moloney murine leukemia virus,
pubmed-meshheading:6601272-Protein Kinases,
pubmed-meshheading:6601272-Rats,
pubmed-meshheading:6601272-Temperature,
pubmed-meshheading:6601272-Viral Proteins
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pubmed:year |
1983
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pubmed:articleTitle |
P85gag-mos encoded by ts110 Moloney murine sarcoma virus has an associated protein kinase activity.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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