Linked Life Data

6601129

Source:http://linkedlifedata.com/resource/pubmed/id/6601129

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1983-5-5
pubmed:abstractText
Lymphocytes that were activated by various means (recovered from an in vivo allograft site, sensitized in mixed lymphocyte culture, or stimulated by Con A in vitro), were extremely motile in vitro when compared to unsensitized lymph node lymphocytes. Allosensitized lymphocytes generated in vitro and in vivo (harvested on days of peak cell-mediated cytotoxicity) were more motile than Con A-activated lymphocytes (harvested on the day of peak thymidine incorporation). Protein (bovine serum albumin, normal mouse serum, or alpha-casein) was chemokinetic for all of the lymphocyte preparations, especially those activated by alloantigen or Con A. When these bulk populations were used, no evidence was found to suggest these materials were chemotactic for activated lymphocytes; the various proteins promoted comparable chemokinetic effects. Treatment of allosensitized lymphocytes generated in vitro with Lyt-2 antisera and complement significantly reduced cell-mediated cytotoxicity, but had no effect on random motility. Conversely, treatment with anti-Lyt-1 antisera and complement impaired the motility of the residual cell population in response to chemokinetic normal mouse serum or alpha-casein. Within the bulk population of lymphocytes allosensitized in vitro, the cells that migrate most quickly are predominantly Thy-1.2, Lyt-1+2-, are not as cytotoxic, and do not incorporate thymidine as actively as the unseparated population. These experiments indicate that subpopulations of lymphocytes demonstrate locomotor heterogeneity. Studies designed to determine if a specific lymphocyte population can manifest directional motility (i.e., chemotaxis) will require the use of various antigen-sensitized lymphocyte clones at defined stages of the cell cycle.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:volume
130
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1610-5
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
The migration of activated murine T lymphocytes in vitro. II. Evidence for differential locomotion of T cell subsets.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.