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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
1984-11-9
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| pubmed:abstractText |
When filtering red blood cells (RBC) resuspended into buffer at constant flow, the pressure-time curve is dependent on erythrocyte deformability (E.D) and also on the plugging of pores by RBC or leukocytes (WBC). To understand the influence of these 3 factors, we used an apparatus with: 1) a constant flow syringe (Bioblock) (1.56 ml/mn); 2) a pressure transducer (Statham P23 ID); 3) a recorder (Gould); 4) a 5 micron polycarbonate filter (Nuclepore) housed in a lucite block according to USAMI & al. (1). Resuspensions of RBC from 4 to 14% haematocrit with 0 to 600 WBC/mm3 were investigated. Results indicate that: 1/ The first inflexion point of the pressure-time curve (initial pressure leading to calculation of beta) is related to the RBC's deformability; 2/beta is (in our experimental conditions) dependent on the WBC number; 3/beta seems very sensitive to deformability changes as shown by rapid increases induced by osmolarity changes.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
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| pubmed:issn |
0891-978X
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
1
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
267-70
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| pubmed:dateRevised |
2004-11-17
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| pubmed:meshHeading | |
| pubmed:year |
1984
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| pubmed:articleTitle |
Assessment of erythrocyte deformability by constant flow filtration technique: analysis of factors influencing the initial pressure.
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| pubmed:publicationType |
Journal Article
|