Linked Life Data

6589453

Source:http://linkedlifedata.com/resource/pubmed/id/6589453

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1984-9-14
pubmed:abstractText
Estradiol 17 beta-dehydrogenase from human placenta was inactivated by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), a specific reagent for modification of carboxyl group in a protein. Inactivation of the dehydrogenase by EDC progressed in time-dependent, concentration-dependent and ionic strength-dependent manners. In the presence of NAD+ or NADP+, the inactivation rate by EDC was markedly accelerated. NMN+ and acetylpyridine-DPN+ also appeared to increase the rate of modification of the dehydrogenase by EDC, but no acceleration of the inactivation was observed in the presence of the reduced forms of those nucleotides. Inactivation by EDC in the presence of NAD+ was accompanied by incorporation of 2 mol of NAD+/mol of dimer (Mr = 68,000) of the enzyme. When the dehydrogenase was incubated with EDC together with estriol, the activity was lost slower than that of control without the steroid. Similarly, the inactivation was prevented by addition of estrone and estradiol as well as aminoestrogens. From those results, we suggest that the carboxyl group essential for the function of estradiol 17 beta-dehydrogenase is buried in the enzyme molecule, and the positive charge of nicotinamide N1 of the oxidized form of the cofactor plays an important role for enhancement of the inactivation by EDC.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-4731
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
59-64
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
The reaction of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide with an essential carboxy group of human placental estradiol 17 beta-dehydrogenase.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't