Linked Life Data

6587406

Source:http://linkedlifedata.com/resource/pubmed/id/6587406

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1984-7-18
pubmed:abstractText
Results obtained from radioimmunoassay (RIA) at various stages of sample processing were compared in order to determine the degree of purification necessary to estimate levels of prostaglandins (PG) and thromboxane B2 (TXB2). In serum, TXB2 levels were closely comparable when assayed directly or after organic extraction and thin layer chromatography (TLC). Although concentrations of 6-keto-PGF1 determined by both methods were correlated, levels were significantly overestimated by the direct method. PGE2 and 6-keto-PGF1 alpha levels measured directly in acetone extracted kidney homogenate were almost identical with kidney homogenates taken through the full extraction procedure and TLC. After indomethacin treatment serum 6-keto-PGF1 alpha and PG levels in kidney homogenate assayed directly were markedly different from extracted values. Thus direct RIA of samples is reliable when prostanoid levels are high in relation to interfering substances, as with serum TXB2 and kidney homogenate 6-keto-PGF1 alpha and PGE2, but not for serum 6-keto-PGF1 alpha or following PG inhibition.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0262-1746
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
55-64
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Validation of the direct radioimmunoassay of prostanoids in serum and kidney.
pubmed:publicationType
Journal Article, Comparative Study