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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1984-6-7
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pubmed:abstractText |
Class I gene products D, L, and R encoded by the D region of the H-2q haplotype were isolated by sequential immunoprecipitation from Nonidet P-40 extracts of B10.AKM murine spleen cells. Primary structural comparisons of these molecules were made by tryptic peptide map analyses and radiochemical amino acid sequence determinations. Partial NH2-terminal radiosequence analyses of the peptides generated by CNBr cleavage of these three molecules defined the Rq molecule as a gene product that is distinct from the Dq and Lq molecules. Comparative tryptic peptide map analyses of [3H]arginine-, [3H]lysine-, or [3H]mannose-labeled molecules were performed to determine if the Dq and Lq molecules could be distinguished. The two molecules were identical in their [3H]lysine-labeled peptides (17 in common) and [3H]mannose-labeled glycopeptides but could be differentiated by their [3H]arginine-labeled peptides (18 of 21 in common). These results suggest an amino acid sequence homology of 99% or greater between the Dq and Lq molecules. These data therefore provide structural evidence for three D region gene products and suggest that their antigenic differences are not due to post-translational glycosylation differences. Thus, the D region of the H-2q haplotype may provide an example of a relatively recent class I gene duplication, indicating that these genes are in a dynamic state of evolution through expansion or contraction of the gene pool.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6585810-11894934,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6585810-4106305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6585810-4108876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6585810-4113868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6585810-4841060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6585810-49386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6585810-505544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6585810-6154735,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6585810-6171504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6585810-6177750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6585810-6191921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6585810-6280871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6585810-6286837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6585810-6571712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6585810-6604086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6585810-6815535,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6585810-6928664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6585810-6952248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6585810-6956576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6585810-6967857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6585810-7011174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6585810-7031135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6585810-7058332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6585810-7118212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6585810-7191868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6585810-7217662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6585810-7231522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6585810-7295646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6585810-7430620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6585810-92183
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
81
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2499-503
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6585810-Amino Acid Sequence,
pubmed-meshheading:6585810-Animals,
pubmed-meshheading:6585810-Antibodies, Monoclonal,
pubmed-meshheading:6585810-Cyanogen Bromide,
pubmed-meshheading:6585810-Genes,
pubmed-meshheading:6585810-H-2 Antigens,
pubmed-meshheading:6585810-Major Histocompatibility Complex,
pubmed-meshheading:6585810-Mice,
pubmed-meshheading:6585810-Mice, Inbred Strains,
pubmed-meshheading:6585810-Papain,
pubmed-meshheading:6585810-Peptide Fragments,
pubmed-meshheading:6585810-Trypsin
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pubmed:year |
1984
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pubmed:articleTitle |
Primary structural evidence that the H-2Dq region encodes at least three distinct gene products: Dq, Lq, and Rq.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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