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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
7
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pubmed:dateCreated |
1983-9-23
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pubmed:abstractText |
Two human osteosarcoma cell lines (TE-85, LM-1) were adapted to grow in serum-free hydrocortisone supplemented medium. Human osteosarcoma associated antigens were detected both on the cell membrane as well as in the concentrated culture medium. TE-85 cells produced bone specific alkaline phosphatase and LM-1 cells produced both bone specific and placental-like alkaline phosphatases when cultured in this medium. Successful proliferation of human osteosarcoma cell lines in serum-free medium is valuable in human osteosarcoma research.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0073-5655
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
515-21
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pubmed:dateRevised |
2009-11-11
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pubmed:meshHeading |
pubmed-meshheading:6575958-Alkaline Phosphatase,
pubmed-meshheading:6575958-Cell Division,
pubmed-meshheading:6575958-Cell Line,
pubmed-meshheading:6575958-Cells, Cultured,
pubmed-meshheading:6575958-Culture Media,
pubmed-meshheading:6575958-Humans,
pubmed-meshheading:6575958-Karyotyping,
pubmed-meshheading:6575958-Microscopy, Electron,
pubmed-meshheading:6575958-Osteosarcoma
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pubmed:year |
1983
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pubmed:articleTitle |
Cultivation of human osteosarcoma cell lines in serum-free hormone supplemented medium.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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