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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1983-8-11
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pubmed:abstractText |
Present evidence suggests that skin is an important organ of prostaglandin metabolism. To clarify its role, the basic kinetics of 15-hydroxyprostaglandin dehydrogenase (PGDH) from rat skin were investigated with either NAD+ of NADP+ as co-substrate. Prostaglandin F2 alpha (PGF2 alpha) and prostaglandin E2 (PGE2) were used as substrates and preliminary studies were made of the inhibitory effects of the reduced co-substrates NADH and NADPH. A radiochemical assay was used in which [3H]PGF2 alpha or [14C]PGE2 were incubated with high-speed supernatant of rat skin homogenates. The substrate and products were then extracted by solvent partition, separated by t.l.c. and quantified by liquid-scintillation counting. At linear reaction rates and at an NAD+ concentration of 10 mM the mean apparent Km for PGF2 alpha was 24 microM with a mean apparent Vmax. of 9.8 nmol/s per litre of reaction mixture. For PGE2 the mean apparent Km was 8 microM, with a mean apparent Vmax, of 2.7 nmol/s per litre of reaction mixture. With NADP+ as a co-substrate at a concentration of 5 mM a mean apparent Km of 23 microM was obtained for PGF2 alpha with a mean apparent Vmax. of 5.2 nmol/s per litre. For PGE2 values of 7.5 microM and 3.0 nmol/s per litre were obtained respectively. These results show that skin contains NAD+- and NADP+-dependent PGDH. An important finding was that the NADP+-linked enzyme gave Km values for PGE2 that were considerably lower than those reported for NADP+-linked PGDH from other tissues. Furthermore, preliminary inhibition studies with the NAD+-linked PGDH system indicate that this enzyme is not only inhibited by NADH, but also by NADPH, a property not previously reported for NAD+-linked PGDH derived from other tissues.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6575778-16744959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6575778-171213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6575778-184496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6575778-20082,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6575778-234431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6575778-236730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6575778-38483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6575778-42948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6575778-4306615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6575778-5543672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6575778-5550521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6575778-5555250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6575778-6813003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6575778-7370004
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
212
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
129-34
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6575778-Animals,
pubmed-meshheading:6575778-Dinoprost,
pubmed-meshheading:6575778-Dinoprostone,
pubmed-meshheading:6575778-Hydroxyprostaglandin Dehydrogenases,
pubmed-meshheading:6575778-Kinetics,
pubmed-meshheading:6575778-NAD,
pubmed-meshheading:6575778-NADP,
pubmed-meshheading:6575778-Prostaglandins E,
pubmed-meshheading:6575778-Prostaglandins F,
pubmed-meshheading:6575778-Rats,
pubmed-meshheading:6575778-Skin,
pubmed-meshheading:6575778-Subcellular Fractions
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pubmed:year |
1983
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pubmed:articleTitle |
Novel prostaglandin dehydrogenase in rat skin.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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