Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1983-6-10
pubmed:abstractText
Spec 1 mRNAs increase 100-fold in abundance per embryo during early sea urchin development. Previous studies indicated an enrichment of this mRNA in ectoderm fractions of gastrulae and plutei. We have determined the precise localization of this mRNA by in situ hybridization techniques. In pluteus larvae, the mRNA is highly restricted to a set of morphologically uniform ectoderm cells in the dorsal part of the embryo. The mRNA is not detectable in other regions of ectoderm or in endoderm and mesoderm. The pattern of localization is already established at the gastrula stage, before these cells are distinguishable by morphological criteria. This pattern of distribution of Spec 1 mRNA is distinct from that of bulk poly(A)+ mRNA. Measurements of the amount of Spec 1 mRNA per embryo and the number of cells containing this RNA indicate that there are about 500 Spec 1 mRNA molecules per cell at the pluteus stage and probably twice as many at the gastrula stage. These results indicate that the sensitivity of the in situ hybridization method allows detection of sequences that comprise approximately equal to 0.05% of the embryo mRNA nucleotides.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6573676-6169002, http://linkedlifedata.com/resource/pubmed/commentcorrection/6573676-6169573, http://linkedlifedata.com/resource/pubmed/commentcorrection/6573676-6178156, http://linkedlifedata.com/resource/pubmed/commentcorrection/6573676-6254074, http://linkedlifedata.com/resource/pubmed/commentcorrection/6573676-6279614, http://linkedlifedata.com/resource/pubmed/commentcorrection/6573676-6284573, http://linkedlifedata.com/resource/pubmed/commentcorrection/6573676-667939, http://linkedlifedata.com/resource/pubmed/commentcorrection/6573676-6933514, http://linkedlifedata.com/resource/pubmed/commentcorrection/6573676-7040372, http://linkedlifedata.com/resource/pubmed/commentcorrection/6573676-7095261, http://linkedlifedata.com/resource/pubmed/commentcorrection/6573676-7195316
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
80
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2656-60
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Localization of a family of MRNAS in a single cell type and its precursors in sea urchin embryos.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.