| pubmed-article:6573664 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6573664 | lifeskim:mentions | umls-concept:C1253959 | lld:lifeskim |
| pubmed-article:6573664 | lifeskim:mentions | umls-concept:C0035700 | lld:lifeskim |
| pubmed-article:6573664 | lifeskim:mentions | umls-concept:C1549781 | lld:lifeskim |
| pubmed-article:6573664 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:6573664 | pubmed:dateCreated | 1983-6-10 | lld:pubmed |
| pubmed-article:6573664 | pubmed:abstractText | A highly charged component can be isolated from a total RNase T2 digest of nuclear polyadenylylated RNA from HeLa cells that is separable from caps by (dihydroxyboryl)aminoethyl-cellulose chromatography. Chemical and enzymatic analyses show that the component contains a 2'-5' phosphodiester bond that creates a branch at the 2'-hydroxyl group of one nucleotide already linked to an adjoining nucleotide through the usual 3'-5' phosphodiester bond. [Formula: see text] This structure was confirmed by analysis of a similar component isolated from nuclease P1 digests of the same nuclear polyadenylylated RNA. Branches occur in roughly 10% of nuclear polyadenylylated RNAs, including those >10S in size, but are absent from cytoplasmic polyadenylylated RNA. Possible implications for branches as intermediates in mRNA processing are discussed. | lld:pubmed |
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| pubmed-article:6573664 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:6573664 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:6573664 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6573664 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:6573664 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:6573664 | pubmed:author | pubmed-author:WallaceJ CJC | lld:pubmed |
| pubmed-article:6573664 | pubmed:author | pubmed-author:EdmondsMM | lld:pubmed |
| pubmed-article:6573664 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6573664 | pubmed:volume | 80 | lld:pubmed |
| pubmed-article:6573664 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6573664 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6573664 | pubmed:pagination | 950-4 | lld:pubmed |
| pubmed-article:6573664 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:6573664 | pubmed:year | 1983 | lld:pubmed |
| pubmed-article:6573664 | pubmed:articleTitle | Polyadenylylated nuclear RNA contains branches. | lld:pubmed |
| pubmed-article:6573664 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6573664 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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