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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1983-6-10
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pubmed:abstractText |
A highly charged component can be isolated from a total RNase T2 digest of nuclear polyadenylylated RNA from HeLa cells that is separable from caps by (dihydroxyboryl)aminoethyl-cellulose chromatography. Chemical and enzymatic analyses show that the component contains a 2'-5' phosphodiester bond that creates a branch at the 2'-hydroxyl group of one nucleotide already linked to an adjoining nucleotide through the usual 3'-5' phosphodiester bond. [Formula: see text] This structure was confirmed by analysis of a similar component isolated from nuclease P1 digests of the same nuclear polyadenylylated RNA. Branches occur in roughly 10% of nuclear polyadenylylated RNAs, including those >10S in size, but are absent from cytoplasmic polyadenylylated RNA. Possible implications for branches as intermediates in mRNA processing are discussed.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6573664-1005114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6573664-14167265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6573664-4369093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6573664-440970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6573664-5961144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6573664-6111419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6573664-6170325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6573664-6176879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6573664-6280184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6573664-6791577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6573664-7266664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6573664-974081
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
80
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
950-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1983
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pubmed:articleTitle |
Polyadenylylated nuclear RNA contains branches.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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