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rdf:type |
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lifeskim:mentions |
umls-concept:C0006556,
umls-concept:C0009015,
umls-concept:C0013032,
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0020792,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0185117,
umls-concept:C1156511,
umls-concept:C2911684
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pubmed:issue |
8
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pubmed:dateCreated |
1983-5-27
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pubmed:abstractText |
mRNA for dopamine beta-hydroxylase [3,4-dihydroxyphenylethylamine, ascorbate:oxygen oxidoreductase (beta-hydroxylating), EC 1.14.17.1] has been partially purified from poly(A)+ mRNA isolated from a rat pheochromocytoma cell line. Shared antigenic determinants between tyrosine hydroxylase and dopamine beta-hydroxylase allowed us to obtain enriched fractions of dopamine beta-hydroxylase mRNA by immunoprecipitating translated mRNA products with tyrosine hydroxylase antisera. The enriched dopamine beta-hydroxylase mRNA was used to synthesize the corresponding cDNAs, which were then cloned in the Pst I site of pBR322. Recombinant colonies were characterized by an in situ colony immunoassay and hybrid-selected translation. In vitro translation of the mRNA selected from one recombinant clone produced a protein of 75,000 daltons that comigrated with authentic dopamine beta-hydroxylase. Partial proteolysis of both authentic dopamine beta-hydroxylase and the protein encoded by the recombinant clone produced identical peptide patterns.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572968-1065897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572968-187693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572968-235542,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572968-291909,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572968-30087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572968-320200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572968-325648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572968-360074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572968-379518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572968-396441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572968-4149845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572968-418881,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572968-4733239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572968-4850204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572968-5013535,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572968-560237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572968-6112744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572968-6153123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572968-6161559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572968-6166921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572968-6179090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572968-6794032,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572968-7346,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572968-823012
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
80
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2161-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6572968-Animals,
pubmed-meshheading:6572968-Cell Line,
pubmed-meshheading:6572968-Cloning, Molecular,
pubmed-meshheading:6572968-DNA,
pubmed-meshheading:6572968-Dopamine beta-Hydroxylase,
pubmed-meshheading:6572968-Gene Expression Regulation,
pubmed-meshheading:6572968-Nucleic Acid Hybridization,
pubmed-meshheading:6572968-Peptide Fragments,
pubmed-meshheading:6572968-RNA, Messenger,
pubmed-meshheading:6572968-Rats
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pubmed:year |
1983
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pubmed:articleTitle |
Genes for catecholamine biosynthesis: cloning by expression and identification of the cDNA for rat dopamine beta-hydroxylase.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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