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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0013879,
umls-concept:C0018042,
umls-concept:C0018330,
umls-concept:C0018353,
umls-concept:C0026649,
umls-concept:C0029246,
umls-concept:C0040715,
umls-concept:C0041348,
umls-concept:C0146894,
umls-concept:C0178719,
umls-concept:C0243071,
umls-concept:C0314672,
umls-concept:C0392760,
umls-concept:C0449774,
umls-concept:C0599718,
umls-concept:C0599813,
umls-concept:C0599893,
umls-concept:C1522702,
umls-concept:C1720154,
umls-concept:C2003941
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pubmed:issue |
7
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pubmed:dateCreated |
1983-5-27
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pubmed:abstractText |
Injection of the alpha, beta-nonhydrolyzable GTP analog, guanosine 5'-[alpha, beta-methylene]triphosphate (pp[CH2]pG) into PtK2, A549, and Swiss 3T3 cells produced dramatic changes in the normal pattern of long radiating microtubules displayed by the cells before injection. Injection of pp[CH2]pG into cells growing in normal medium resulted in the formation of microtubule bundles resistant to depolymerization by Colcemid and calcium. Cells injected with pp[CH2]pG after incubation with Colcemid for 2 hr showed polymerization of tubulin into long wavy ribbons within 2 hr after injection. Removal of Colcemid 1 hr after the injection of pp[CH2]pG resulted in assembly of tubulin into short single randomly oriented microtubules. All cells injected with pp[CH2]pG showed impeded translocation and restriction or absence of intracellular movement. pp[CH2]pG also prevented the fragmentation of Golgi elements in A549 cells treated with Colcemid. Cells first treated with Colcemid and then injected with pp[CH2]pG failed to reassemble the Golgi elements after the removal of Colcemid. Cells in intimate membrane contact with cells injected with pp[CH2]pG showed similar changes in microtubule polymerization, cell movement, and organization of Golgi elements.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572952-14482095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572952-174086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572952-200938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572952-341163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572952-4280448,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572952-4626639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572952-537060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572952-5700666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572952-620419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572952-6787115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572952-6811596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572952-6893454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572952-7391061,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572952-7462348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572952-77736
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
80
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1938-41
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1983
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pubmed:articleTitle |
Cells injected with guanosine 5'-[alpha, beta-methylene]triphosphate, an alpha, beta-nonhydrolyzable analog of GTP, show anomalous patterns of tubulin polymerization affecting cell translocation, intracellular movement, and the organization of Golgi elements.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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