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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1983-3-24
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pubmed:abstractText |
Two 19-base-long oligonucleotides were synthesized, one complementary to the normal human beta-globin gene (beta A) and one complementary to the sickle cell beta-globin gene (beta S). The nonadecanucleotides were radioactively labeled and used as probes in DNA hybridization. Under appropriate hybridization conditions, these probes can be used to distinguish the beta A gene from the beta S allele. The DNA from individuals homozygous for the normal beta-globin gene (beta A beta A) only hybridized with the beta A specific probe; the DNA from those homozygous for the sickle cell beta-globin gene (beta S beta S) only hybridized with the beta S specific probe. The DNA from heterozygous individuals (beta A beta S) hybridized with both probes. This allele-specific hybridization behavior of oligonucleotides provides a general method for diagnosis of any genetic disease which involves a point mutation in the DNA sequence of a single-copy gene.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572002-10793670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572002-1103090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572002-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572002-15395398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572002-158748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572002-291943,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572002-4868544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572002-6155216,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572002-6171820,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572002-6176866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572002-6176867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572002-6254663,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572002-6254664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572002-6276651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572002-6280057,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572002-6285354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572002-635620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572002-6452087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572002-68958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572002-6954478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572002-7031661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572002-7070526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572002-7232206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572002-7279669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572002-728996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572002-743594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572002-7438203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6572002-909565
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
80
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
278-82
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1983
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pubmed:articleTitle |
Detection of sickle cell beta S-globin allele by hybridization with synthetic oligonucleotides.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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