Linked Life Data

6546920

Source:http://linkedlifedata.com/resource/pubmed/id/6546920

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1984-6-5
pubmed:abstractText
ADvF11 (F11), a Chinese Hamster Ovary (CHO) cell variant, is defective in its ability to adhere to fibronectin (Fn)-coated substrata but will adhere to substrata coated with poly-L-lysine, conA or extracellular matrix (ECM) [1]. We have observed that both F11 and CHO wild-type (WT) cells were able to bind 3H-Fn beads in a similar manner; however, only WT cells and not F11 cells aggregate in the presence of Fn beads. Both cell types aggregated similarly in the presence of lectins. Fn-bead-mediated aggregation was blocked by low temperature and aggregation did not occur when formaldehyde-fixed WT cells were used. Colchicine, tetracaine and cytochalasin B were not effective in blocking aggregation induced by Fn beads. These results suggest that: 1. Both WT and F11 cells have surface membrane-binding sites for Fn. 2. The aggregation defect in F11 cells is distal to the initial interaction between the cell surface and Fn, but proximal to the cytoskeletal rearrangements required for cell adhesion.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0014-4827
pubmed:author
pubmed:issnType
Print
pubmed:volume
152
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
127-33
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
CHO cell aggregation induced by fibronectin-coated beads. Differences between wild-type and adhesion-variant cells.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.