Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1984-4-10
|
| pubmed:abstractText |
A cDNA clone for mouse apolipoprotein E has been identified from a mouse liver cDNA library by a combination of differential colony hybridization and hybrid selection-translation. The identity of the clone was unambiguously established by partial sequencing and comparison with human apolipoprotein E nucleotide and amino acid sequences. In conjunction with an in vitro translation assay for apolipoprotein E, the clone has been used to examine the relative levels of apolipoprotein E mRNA in various tissues of the mouse and the regulation of apolipoprotein E synthesis in response to a diet rich in saturated fat and cholesterol. In the tissues examined, the clone was found to hybridize to a polyadenylated RNA species of approximately 1400 nucleotides. Of the tissues involved in lipoprotein synthesis, liver is very rich (about 1% of total) in apolipoprotein E mRNA while intestine contains only trace amounts. Appreciable levels of active apolipoprotein E mRNA (up to 10% of that in liver) are also detected in peripheral tissues not associated with lipoprotein synthesis, including lung, kidney, spleen, and heart. Thus, extrahepatic apolipoprotein E synthesis may contribute significantly to the levels present in plasma, and a possible function in "reverse cholesterol transport" is considered. When mice were placed on a high lipid diet there was no discernible change in the level of apolipoprotein E mRNA in liver or intestine, although the level of the circulating protein increased about 3-fold. We conclude that in mice the effect of diet on apolipoprotein E levels in blood does not result from induction of mRNA in these tissues.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
25
|
| pubmed:volume |
259
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2100-7
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:6546570-Amino Acid Sequence,
pubmed-meshheading:6546570-Animals,
pubmed-meshheading:6546570-Apolipoproteins,
pubmed-meshheading:6546570-Apolipoproteins E,
pubmed-meshheading:6546570-Base Sequence,
pubmed-meshheading:6546570-Cloning, Molecular,
pubmed-meshheading:6546570-DNA,
pubmed-meshheading:6546570-Liver,
pubmed-meshheading:6546570-Mice,
pubmed-meshheading:6546570-Mice, Inbred BALB C,
pubmed-meshheading:6546570-Mice, Inbred C57BL,
pubmed-meshheading:6546570-Nucleic Acid Hybridization,
pubmed-meshheading:6546570-Plasmids,
pubmed-meshheading:6546570-Protein Biosynthesis,
pubmed-meshheading:6546570-RNA, Messenger,
pubmed-meshheading:6546570-Rabbits,
pubmed-meshheading:6546570-Rats,
pubmed-meshheading:6546570-Reticulocytes,
pubmed-meshheading:6546570-Transcription, Genetic
|
| pubmed:year |
1984
|
| pubmed:articleTitle |
Cloning and regulation of messenger RNA for mouse apolipoprotein E.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|