Linked Life Data

6544886

Source:http://linkedlifedata.com/resource/pubmed/id/6544886

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1985-12-27
pubmed:abstractText
Sea urchin egg cortices, isolated on polylysine-coated cover glasses by the method of V.D. Vacquier (1975, Dev. Biol. 43, 62-74), have been fixed with 2% glutaraldehyde, critical point-dried, and rotary shadowed with platinum-carbon. The replicas produced show that the isolated cortex consists of a plasma membrane having a regular array of microvillar evaginations each containing a filamentous core. Attached to the plasma membrane is a densely packed layer of cortical granules; each granule is linked to the plasma membrane and to four to six adjacent granules by fine, 6-nm-diameter filaments. Between the plasma membrane and cortical granule layer runs a tubular endoplasmic reticulum consisting of bulb-like varicosities joined in chains that are arranged in a network of polygons. Interspersed among the cortical granules are occasional spherical organelles which correspond in size to osmiophilic granules seen in the intact egg cortex. Addition of 1000 microM or 50 microM Ca2+ to the cortex initiates fusion of individual granules with the plasma membrane, as well as extensive fusion between granules, producing extended, branched exocytic pockets. Frequently, fusion patterns are observed that suggest a propagation of granule fusion outward from one or more foci within a single cortex.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-5320
pubmed:author
pubmed:issnType
Print
pubmed:volume
89
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
198-211
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Exocytosis in vitro: ultrastructure of the isolated sea urchin egg cortex as seen in platinum replicas.
pubmed:publicationType
Journal Article, In Vitro