Linked Life Data

6544863

Source:http://linkedlifedata.com/resource/pubmed/id/6544863

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1985-12-26
pubmed:abstractText
Hydroxyethylmethacrylate (HEMA) hydrogels have a peculiar crater-like topography which renders them ideal for studying cell-to-substrate contact formation. The hydrogel is quite suitable for such studies since it is transparent, which allows for light microscopic observations, it is not toxic, and it will support cell growth only when an additional protein component such as collagen is integrated into its surface. Cultured rabbit aortic smooth muscle cells (SMC) were grown on collagen-HEMA hydrogels, and the ultrastructure of developing cell attachment sites was studied. By 3 hr after cell seeding, both the rounded and the spreading SMC appeared anchored to the hydrogel via extracellular connective tissue-like material. The fully formed attachment site present at 5-8 days was characterized by large bundles of intracellular myofilaments inserting onto areas of increased electron density along the plasmalemmal membrane; large amounts of extracellular connective tissue-like material also appeared attached to the areas of increased electron density. Fully formed cell substratum attachment sites were not observed when either elastin-HEMA hydrogels or hydrogels polymerized in the absence of protein were employed.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-5320
pubmed:author
pubmed:issnType
Print
pubmed:volume
86
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
252-61
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Ultrastructural studies of attachment site formation in aortic smooth muscle cells cultured on collagen-hydroxyethylmethacrylate hydrogels.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.