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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1985-12-20
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pubmed:abstractText |
Leukemic cells were obtained from 12 patients with acute nonlymphocytic leukemia and 2 patients with acute lymphocytic leukemia. The specimens were incubated with a range of concentrations of adriamycin (Adr) for different periods of time and then analyzed by flow cytometry. For incubations of up to 5 h, only cells exposed to 5 or more micrograms of Adr ml-1 became fluorescent while cells incubated with 1 microgram ml-1 for 24 h became fluorescent. Fluorescence decreased when the cells were washed and further decreased when the cells were incubated in Adr-free media. The cells from different patients exhibited a wide range of Adr uptakes and retentions. Simultaneous analysis of right angle light scatter and fluorescence intensity demonstrated that there may be subpopulations of cells whose Adr uptake and/or retention differ from the weighted mean population values. With this method rare cells which fail to take up Adr can be recognized.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0736-0118
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
43-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading | |
pubmed:year |
1984
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pubmed:articleTitle |
Uptake of adriamycin by human leukemic cells as measured by flow cytometry.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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