| pubmed-article:6537820 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6537820 | lifeskim:mentions | umls-concept:C0025011 | lld:lifeskim |
| pubmed-article:6537820 | lifeskim:mentions | umls-concept:C0035668 | lld:lifeskim |
| pubmed-article:6537820 | lifeskim:mentions | umls-concept:C0178719 | lld:lifeskim |
| pubmed-article:6537820 | lifeskim:mentions | umls-concept:C0178774 | lld:lifeskim |
| pubmed-article:6537820 | lifeskim:mentions | umls-concept:C0204727 | lld:lifeskim |
| pubmed-article:6537820 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:6537820 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:6537820 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:6537820 | pubmed:dateCreated | 1984-2-15 | lld:pubmed |
| pubmed-article:6537820 | pubmed:abstractText | Protocols have been established for the preparation of large amounts of pure measles virus intracellular nucleocapsids. As a result, it has been possible to routinely achieve nucleocapsid RNA yields of approximately 200 micrograms (from approximately 5 X 10(8) infected cells). Electrophoretic analysis of this RNA under denaturing conditions revealed a single species whose mass was estimated at approximately 4.8 X 10(6) daltons. Electron microscopic assessment of nucleocapsid RNA contour lengths corroborated the electrophoretic size determination. Total nucleocapsid RNA was shown to contain both negative- and positive-stranded species distributed in a ratio of 2 to 3 genome polarity molecules for each antigenome RNA. Hybridization studies established that all of the virus-specified polyadenylated RNAs were encoded by the negative-stranded nucleocapsid RNA and, therefore, that this nucleocapsid RNA was the measles genome. Examination of the measles virus-specified, polyadenylated transcription products by HCHO-agarose gel electrophoresis revealed at least nine distinct RNA species (rather than the six predicted measles mRNAs). The significance of these observations is discussed. | lld:pubmed |
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| pubmed-article:6537820 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:6537820 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:6537820 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6537820 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:6537820 | pubmed:issn | 0022-538X | lld:pubmed |
| pubmed-article:6537820 | pubmed:author | pubmed-author:CookK AKA | lld:pubmed |
| pubmed-article:6537820 | pubmed:author | pubmed-author:UdemS ASA | lld:pubmed |
| pubmed-article:6537820 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6537820 | pubmed:volume | 49 | lld:pubmed |
| pubmed-article:6537820 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6537820 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6537820 | pubmed:pagination | 57-65 | lld:pubmed |
| pubmed-article:6537820 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:6537820 | pubmed:year | 1984 | lld:pubmed |
| pubmed-article:6537820 | pubmed:articleTitle | Isolation and characterization of measles virus intracellular nucleocapsid RNA. | lld:pubmed |
| pubmed-article:6537820 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6537820 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:6537820 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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