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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1984-2-15
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pubmed:abstractText |
Protocols have been established for the preparation of large amounts of pure measles virus intracellular nucleocapsids. As a result, it has been possible to routinely achieve nucleocapsid RNA yields of approximately 200 micrograms (from approximately 5 X 10(8) infected cells). Electrophoretic analysis of this RNA under denaturing conditions revealed a single species whose mass was estimated at approximately 4.8 X 10(6) daltons. Electron microscopic assessment of nucleocapsid RNA contour lengths corroborated the electrophoretic size determination. Total nucleocapsid RNA was shown to contain both negative- and positive-stranded species distributed in a ratio of 2 to 3 genome polarity molecules for each antigenome RNA. Hybridization studies established that all of the virus-specified polyadenylated RNAs were encoded by the negative-stranded nucleocapsid RNA and, therefore, that this nucleocapsid RNA was the measles genome. Examination of the measles virus-specified, polyadenylated transcription products by HCHO-agarose gel electrophoresis revealed at least nine distinct RNA species (rather than the six predicted measles mRNAs). The significance of these observations is discussed.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6537820-113558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6537820-119717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6537820-166498,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6537820-172654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6537820-182384,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/6537820-219606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6537820-352962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6537820-4291924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6537820-4305631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6537820-4315961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6537820-4359293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6537820-4365322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6537820-4504350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6537820-4977468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6537820-501804,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6537820-513197,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6537820-5237493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6537820-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6537820-5550792,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/6537820-911786
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
49
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
57-65
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6537820-Capsid,
pubmed-meshheading:6537820-Genes, Viral,
pubmed-meshheading:6537820-HeLa Cells,
pubmed-meshheading:6537820-Humans,
pubmed-meshheading:6537820-Measles virus,
pubmed-meshheading:6537820-Microscopy, Electron,
pubmed-meshheading:6537820-Molecular Weight,
pubmed-meshheading:6537820-RNA, Messenger,
pubmed-meshheading:6537820-RNA, Viral,
pubmed-meshheading:6537820-Virus Replication
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pubmed:year |
1984
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pubmed:articleTitle |
Isolation and characterization of measles virus intracellular nucleocapsid RNA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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