Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1985-3-21
|
| pubmed:abstractText |
The cobalt-lysine method for tract tracing in the vertebrate CNS has been shortened and simplified to the following steps: (1) the cobalt-lysine is applied to the nerve or tract being studied; (2) the animal is perfused 1-2 days later by the following sequence of solutions: (a) phosphate-buffered ammonium sulfide, (b) phosphate buffer alone and (c) phosphate-buffered glutaraldehyde; (3) the brain is exposed to the fixative overnight and left in 30% sugar another night; (4) the brain is then frozen and cut on a cryostat; the sections being collected directly on slides; (5) the mounted sections are then put in an intensification solution before dehydration, clearing and coverslipping.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0304-3940
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
26
|
| pubmed:volume |
51
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
315-8
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading | |
| pubmed:year |
1984
|
| pubmed:articleTitle |
A simplified cobalt-lysine method for tracing axon trajectories in the central nervous system of vertebrates.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|