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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1985-3-14
|
| pubmed:abstractText |
A procedure based on automated amino acid analysis has been developed to simultaneously quantify 1-methylhistidine (1-MH), 3-methylhistidine (3-MH), tyrosine, phenylalanine, tryptophan, lysine, histidine and arginine levels in human and rat urines. Deproteinized urine samples containing amino acids in the range 1-10 nmol were analyzed using single-column methodology with ninhydrin detection. Standard curves produced correlation coefficients greater than or equal to 0.99 with duplicate analyses agreeing to within +/- 1.9%. Quantitative recovery was ensured by using L-alpha-amino-beta-guanidinopropionic acid as an internal standard. Elution was accomplished in less than 90 min at pH 5.7 with sodium citrate buffers at 45 degrees C and 65 degrees C. Since 3-MH in the rat is acetylated at the alpha-amino group, rat, but not human, urine ultrafiltrates required acid hydrolysis prior to analysis. The utility of the technique of analysis of 1-MH and 3-MH in human urine was demonstrated for an adult male on a meat-free diet for 21 days; urinary excretion rates for 3-MH and 1-MH were determined to be 3.06 +/- 0.10 and 0.72 +/- 0.07 mumol/kg body mass/day, respectively. The technique was also used to measure the effect of disuse atrophy of rat skeletal muscle which induced a 40-60% increase in 3-MH. The procedure is also highly suited for measurement of urinary aromatic and/or basic amino acids.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-methylhistidine,
http://linkedlifedata.com/resource/pubmed/chemical/3-methylhistidine,
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Histidine,
http://linkedlifedata.com/resource/pubmed/chemical/Methylhistidines,
http://linkedlifedata.com/resource/pubmed/chemical/Phenylalanine,
http://linkedlifedata.com/resource/pubmed/chemical/Tryptophan,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0021-9673
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
28
|
| pubmed:volume |
311
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
267-76
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:6520174-Amino Acids,
pubmed-meshheading:6520174-Animals,
pubmed-meshheading:6520174-Chromatography, Ion Exchange,
pubmed-meshheading:6520174-Histidine,
pubmed-meshheading:6520174-Humans,
pubmed-meshheading:6520174-Hydrogen-Ion Concentration,
pubmed-meshheading:6520174-Hydrolysis,
pubmed-meshheading:6520174-Immobilization,
pubmed-meshheading:6520174-Male,
pubmed-meshheading:6520174-Methylhistidines,
pubmed-meshheading:6520174-Phenylalanine,
pubmed-meshheading:6520174-Rats,
pubmed-meshheading:6520174-Rats, Inbred Strains,
pubmed-meshheading:6520174-Species Specificity,
pubmed-meshheading:6520174-Temperature,
pubmed-meshheading:6520174-Tryptophan,
pubmed-meshheading:6520174-Tyrosine,
pubmed-meshheading:6520174-Ultrafiltration
|
| pubmed:year |
1984
|
| pubmed:articleTitle |
Analysis of 1- and 3-methylhistidines, aromatic and basic amino acids in rat and human urine.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|