Linked Life Data

6513555

Source:http://linkedlifedata.com/resource/pubmed/id/6513555

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1985-2-21
pubmed:abstractText
Total rat uterine mRNA was isolated following 1,2 or 3 days of estradiol, 3 days of progesterone, or 3 days of progesterone and estradiol treatment of immature animals. The mRNA translation products were analyzed by 2-dimensional electrophoresis. The results demonstrate that a number of mRNA products were affected by varying the hormonal conditions. Estradiol consistently caused an increase in mRNA in vitro translation products corresponding to polypeptides with mol. wt of 40,000 Daltons (p40), 59,000 Daltons (p59). A polypeptide with a mol. wt of 37,000 Daltons (p37) consistently decreases in concentration following estradiol administration. Progesterone, alone, caused a decrease in p37, p59, and a slight increase in p40. When progesterone was combined with estradiol we detected translation product levels similar to those seen following 1 day of estradiol treatment. Thus, the change in mRNA populations as a response of the immature rat uterus to estradiol and progesterone can be utilized to characterize the tissue's response to these hormones. This type of study also provides a method for detecting gene products which may possibly be utilized as potential markers to investigate estrogen and progesterone action.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-4731
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
571-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Steroidal regulation of rat uterine in vitro mRNA translation products.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't