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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1985-1-17
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pubmed:abstractText |
A monoclonal antibody to the double-stranded polyribonucleotide complex poly(A) . poly(U) was derived from the fusion of spleen cells from immunized DBA/2 mice and the P3 X X63-Ag8 plasma cytoma. Specificity studies using radioimmunoassays showed that the anti-poly(A) . poly(U) does not cross-react with single-stranded polyribonucleotides. RNA X DNA hybrids or DNAs. In addition to RNA duplexes associating adenine and uracil, it recognizes synthetic poly(I) . poly(C) and naturally occurring reovirus RNA. It is thus directed against a conformational epitope with an absolute requirement for two polyribose phosphate chains. However, the antibody does not cross-react with poly(G) . poly(C) and is therefore able to distinguish between RNA double helices.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0161-5890
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
21
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
939-44
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:6504052-Animals,
pubmed-meshheading:6504052-Antibodies, Monoclonal,
pubmed-meshheading:6504052-Antibody Specificity,
pubmed-meshheading:6504052-Binding, Competitive,
pubmed-meshheading:6504052-Cells, Cultured,
pubmed-meshheading:6504052-Cross Reactions,
pubmed-meshheading:6504052-Female,
pubmed-meshheading:6504052-Mice,
pubmed-meshheading:6504052-Mice, Inbred BALB C,
pubmed-meshheading:6504052-Mice, Inbred DBA,
pubmed-meshheading:6504052-Molecular Conformation,
pubmed-meshheading:6504052-Plasmacytoma,
pubmed-meshheading:6504052-Poly A-U,
pubmed-meshheading:6504052-Poly C,
pubmed-meshheading:6504052-Poly G,
pubmed-meshheading:6504052-Poly I-C,
pubmed-meshheading:6504052-Poly U,
pubmed-meshheading:6504052-RNA, Viral,
pubmed-meshheading:6504052-Radioimmunoassay,
pubmed-meshheading:6504052-Spleen
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pubmed:year |
1984
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pubmed:articleTitle |
A monoclonal antibody to the double-stranded polyribonucleotide complex poly(A) X poly(U).
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pubmed:publicationType |
Journal Article
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