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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
1985-1-10
|
| pubmed:abstractText |
From auxotrophic and idiotrophic mutants of Streptomyces fradiae (tylosin producer) and Streptomyces sp. AM 4900 (pikromycin producer) or Streptomyces narbonensis (narbomycin producer), prototrophic fusants were obtained at a low frequency by the protoplast fusion technique. In the cross of S. fradiae 261-27E (mycaminose-idiotroph, ilv) and Streptomyces sp. AM 4900 N3-4, (pikronolide-idiotroph, arg), an unstable prototrophic fusant, strain No. 14, produced a macrolide antibiotic which was not produced by the wild type, parent strains, and the productivity was lost within a few times transfer. It was concluded that the fusant was not a recombinant, but a heterokaryon. On the other hand, relatively stable fusants were obtained from the cross of S. fradiae TBM (mycaminose-idiotroph, met) and S. narbonensis NA12US3 (narbonolide-idiotroph, his, str) at a frequency of 3.2 X 10(-5). One of the prototrophic fusants produced narbomycin, which is believed to be due to a result of interspecific recombination.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0021-8820
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
37
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1224-30
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading | |
| pubmed:year |
1984
|
| pubmed:articleTitle |
Interspecific protoplast fusion among macrolide-producing streptomycetes.
|
| pubmed:publicationType |
Journal Article
|