| pubmed-article:6500705 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6500705 | lifeskim:mentions | umls-concept:C0034493 | lld:lifeskim |
| pubmed-article:6500705 | lifeskim:mentions | umls-concept:C0027950 | lld:lifeskim |
| pubmed-article:6500705 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:6500705 | lifeskim:mentions | umls-concept:C0023503 | lld:lifeskim |
| pubmed-article:6500705 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:6500705 | pubmed:dateCreated | 1984-12-31 | lld:pubmed |
| pubmed-article:6500705 | pubmed:abstractText | The leukocytotoxic toxin pseudomonal leukocidin, produced by Pseudomonas aeruginosa, was radioiodinated with chloramine-T reagent. Binding of [125I]leukocidin to rabbit polymorphonuclear leukocytes was found to be concentration dependent at 37 degrees C. A Scatchard plot of binding data was linear, indicating that leukocidin binds to a single population of sites. The dissociation constant, KD, was calculated from the Scatchard plot to be 2.5 X 10(-7) M, and the number of binding sites per leukocyte was approximately 4.4 X 10(5). Unlabeled leukocidin or antileukocidin antibody reduced the binding of [125I]leukocidin to the leukocytes. A leukocidin-binding protein was extracted from rabbit polymorphonuclear leukocytes with Triton X-100 and purified by leukocidin-Sepharose 4B affinity column chromatography. Approximately 60 micrograms of binding protein was obtained from 8.1 mg of material extracted from the leukocytes. The binding protein had a molecular weight of about 50,000 as shown by sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis and staining with silver nitrate. Under nondenaturing conditions, its molecular weight was also about 50,000, as shown by gel filtration-Sephadex G-200 chromatography. The 50,000-dalton protein purified in this way from rabbit polymorphonuclear leukocytes competitively inhibited the binding of leukocidin to leukocytes and inactivated leukocidin activity. With equimolar amounts of the 50,000-dalton protein and leukocidin, up to 90% inactivation of leukocidin was observed. | lld:pubmed |
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| pubmed-article:6500705 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6500705 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6500705 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:6500705 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6500705 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6500705 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6500705 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:6500705 | pubmed:issn | 0019-9567 | lld:pubmed |
| pubmed-article:6500705 | pubmed:author | pubmed-author:HirayamaTT | lld:pubmed |
| pubmed-article:6500705 | pubmed:author | pubmed-author:KatzDD | lld:pubmed |
| pubmed-article:6500705 | pubmed:author | pubmed-author:NodaMM | lld:pubmed |
| pubmed-article:6500705 | pubmed:author | pubmed-author:MatsudaFF | lld:pubmed |
| pubmed-article:6500705 | pubmed:author | pubmed-author:NagamoriMM | lld:pubmed |
| pubmed-article:6500705 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6500705 | pubmed:volume | 46 | lld:pubmed |
| pubmed-article:6500705 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6500705 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6500705 | pubmed:pagination | 631-4 | lld:pubmed |
| pubmed-article:6500705 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:6500705 | pubmed:meshHeading | pubmed-meshheading:6500705-... | lld:pubmed |
| pubmed-article:6500705 | pubmed:meshHeading | pubmed-meshheading:6500705-... | lld:pubmed |
| pubmed-article:6500705 | pubmed:meshHeading | pubmed-meshheading:6500705-... | lld:pubmed |
| pubmed-article:6500705 | pubmed:meshHeading | pubmed-meshheading:6500705-... | lld:pubmed |
| pubmed-article:6500705 | pubmed:meshHeading | pubmed-meshheading:6500705-... | lld:pubmed |
| pubmed-article:6500705 | pubmed:meshHeading | pubmed-meshheading:6500705-... | lld:pubmed |
| pubmed-article:6500705 | pubmed:meshHeading | pubmed-meshheading:6500705-... | lld:pubmed |
| pubmed-article:6500705 | pubmed:year | 1984 | lld:pubmed |
| pubmed-article:6500705 | pubmed:articleTitle | Binding of pseudomonal leukocidin to rabbit polymorphonuclear leukocytes. | lld:pubmed |
| pubmed-article:6500705 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6500705 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6500705 | lld:pubmed |
