Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1984-12-31
|
| pubmed:abstractText |
The leukocytotoxic toxin pseudomonal leukocidin, produced by Pseudomonas aeruginosa, was radioiodinated with chloramine-T reagent. Binding of [125I]leukocidin to rabbit polymorphonuclear leukocytes was found to be concentration dependent at 37 degrees C. A Scatchard plot of binding data was linear, indicating that leukocidin binds to a single population of sites. The dissociation constant, KD, was calculated from the Scatchard plot to be 2.5 X 10(-7) M, and the number of binding sites per leukocyte was approximately 4.4 X 10(5). Unlabeled leukocidin or antileukocidin antibody reduced the binding of [125I]leukocidin to the leukocytes. A leukocidin-binding protein was extracted from rabbit polymorphonuclear leukocytes with Triton X-100 and purified by leukocidin-Sepharose 4B affinity column chromatography. Approximately 60 micrograms of binding protein was obtained from 8.1 mg of material extracted from the leukocytes. The binding protein had a molecular weight of about 50,000 as shown by sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis and staining with silver nitrate. Under nondenaturing conditions, its molecular weight was also about 50,000, as shown by gel filtration-Sephadex G-200 chromatography. The 50,000-dalton protein purified in this way from rabbit polymorphonuclear leukocytes competitively inhibited the binding of leukocidin to leukocytes and inactivated leukocidin activity. With equimolar amounts of the 50,000-dalton protein and leukocidin, up to 90% inactivation of leukocidin was observed.
|
| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6500705-117571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6500705-14097352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6500705-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6500705-4685374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6500705-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6500705-6162199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6500705-6324749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6500705-6415370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6500705-6418658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6500705-6814956,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6500705-6862019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6500705-818018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6500705-819371,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6500705-819616,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6500705-98070
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0019-9567
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
46
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
631-4
|
| pubmed:dateRevised |
2009-11-18
|
| pubmed:meshHeading | |
| pubmed:year |
1984
|
| pubmed:articleTitle |
Binding of pseudomonal leukocidin to rabbit polymorphonuclear leukocytes.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|