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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12 Pt 1
|
| pubmed:dateCreated |
1985-1-3
|
| pubmed:abstractText |
Monoclonal antibody B72.3 binds to a glycoprotein complex with a molecular weight of 220,000 to 400,000. B72.3 reacts with approximately 50% of human mammary carcinomas and to 80% of the colon carcinomas tested but does not react appreciably with normal mammary tissue, with normal colon, or to a variety of normal adult human tissues tested using immunohistochemical techniques. B72.3 immunoglobulin G was purified and radiolabeled with 125I without significant loss in its reactivity to tumor extracts. The radiolabeled B72.3 immunoglobulin G was shown to efficiently localize human colon carcinoma xenografts in athymic mice. Tumor:tissue ratios of the localized antibody rose over the 7-day period studied, with tumor:liver, tumor:spleen, or tumor:kidney ratios of approximately 18:1 at Day 7 and a tumor:blood ratio of approximately 5:1 at Day 7. Tumor:muscle or tumor:brain ratios rose to over 100:1. The amount of radioactivity in the tumor increased for the first 2 days postinoculation of antibody and stayed constant over a 19-day period of study. Thus, there was no appreciable loss of radioiodine from the tumor over the study interval. No localization was seen in mice bearing a B72.3 antigen-negative human melanoma xenograft or with an isotype-identical control immunoglobulin G in mice bearing colon tumor xenografts. Gamma camera imaging with a pinhole collimator confirmed the ability of the radiolabeled antibody to detect the presence of colon carcinoma xenografts less than 0.5 cm in diameter over a 19-day period. The potential use of this system as a model for radioimmunotherapy will be discussed.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0008-5472
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
44
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
5744-51
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:6498836-Animals,
pubmed-meshheading:6498836-Antibodies, Monoclonal,
pubmed-meshheading:6498836-Antigens, Neoplasm,
pubmed-meshheading:6498836-Cell Line,
pubmed-meshheading:6498836-Colonic Neoplasms,
pubmed-meshheading:6498836-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:6498836-Female,
pubmed-meshheading:6498836-Glycoproteins,
pubmed-meshheading:6498836-Humans,
pubmed-meshheading:6498836-Iodine Radioisotopes,
pubmed-meshheading:6498836-Mice,
pubmed-meshheading:6498836-Mice, Nude,
pubmed-meshheading:6498836-Neoplasm Transplantation,
pubmed-meshheading:6498836-Radioimmunoassay,
pubmed-meshheading:6498836-Transplantation, Heterologous
|
| pubmed:year |
1984
|
| pubmed:articleTitle |
Prolonged binding of a radiolabeled monoclonal antibody (B72.3) used for the in situ radioimmunodetection of human colon carcinoma xenografts.
|
| pubmed:publicationType |
Journal Article
|