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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
8
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pubmed:dateCreated |
1984-11-13
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pubmed:abstractText |
Relationships between thaw rate, thaw bath time, and initial bath and final seminal temperature with coefficients of determination .99 and .97 were: bath time = -.01 + 220.25(1/thaw rate); initial bath temperature = final seminal temperature - 7.29 + 390.05 (1/bath time). Ejaculates from 10 bulls were split and processed in egg yolk-citrate-glycerol, egg yolk-Tris-glycerol, and whole milk-glycerol. All semen was packaged and frozen in .5-ml French straws at -196 degrees C. Sixteen thaw treatments consisted of factorial combinations of four final seminal temperatures and four thaw rates. Treatments were assessed by post-thaw acrosomal integrity after 3-h 37 degrees C incubation. Seminal quality improved with increasing final seminal temperature up to 31 degrees C and did not differ between 31 and 44 degrees C for any of the extenders. A slow thaw rate (3 degrees C/s) resulted in inferior quality for all extenders, and rates 11, 19, and 27 degrees C/s resulted in similar quality for citrate and milk extended semen. Acrosomal integrity was most for 19 degrees C/s in Tris extended semen. A significant factorial interaction existed for Tris and milk extended semen. Predicted acrosomal response of 57.7% across all extenders was at optimum final seminal temperature and thaw rate 37 degrees C and 18 degrees C/s. Bath temperature and bath time determine optimum thaw rate and final temperature of semen packaged in French straws and thus maximize seminal quality.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-0302
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
67
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1806-12
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:6480962-Acrosome,
pubmed-meshheading:6480962-Animals,
pubmed-meshheading:6480962-Cattle,
pubmed-meshheading:6480962-Citrates,
pubmed-meshheading:6480962-Citric Acid,
pubmed-meshheading:6480962-Fertility,
pubmed-meshheading:6480962-Freezing,
pubmed-meshheading:6480962-Male,
pubmed-meshheading:6480962-Milk,
pubmed-meshheading:6480962-Models, Biological,
pubmed-meshheading:6480962-Regression Analysis,
pubmed-meshheading:6480962-Semen,
pubmed-meshheading:6480962-Semen Preservation,
pubmed-meshheading:6480962-Temperature,
pubmed-meshheading:6480962-Time Factors,
pubmed-meshheading:6480962-Tromethamine
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pubmed:year |
1984
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pubmed:articleTitle |
Relationship between final temperature, thaw rate, and quality of bovine semen.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|