Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1984-11-13
pubmed:abstractText
Relationships between thaw rate, thaw bath time, and initial bath and final seminal temperature with coefficients of determination .99 and .97 were: bath time = -.01 + 220.25(1/thaw rate); initial bath temperature = final seminal temperature - 7.29 + 390.05 (1/bath time). Ejaculates from 10 bulls were split and processed in egg yolk-citrate-glycerol, egg yolk-Tris-glycerol, and whole milk-glycerol. All semen was packaged and frozen in .5-ml French straws at -196 degrees C. Sixteen thaw treatments consisted of factorial combinations of four final seminal temperatures and four thaw rates. Treatments were assessed by post-thaw acrosomal integrity after 3-h 37 degrees C incubation. Seminal quality improved with increasing final seminal temperature up to 31 degrees C and did not differ between 31 and 44 degrees C for any of the extenders. A slow thaw rate (3 degrees C/s) resulted in inferior quality for all extenders, and rates 11, 19, and 27 degrees C/s resulted in similar quality for citrate and milk extended semen. Acrosomal integrity was most for 19 degrees C/s in Tris extended semen. A significant factorial interaction existed for Tris and milk extended semen. Predicted acrosomal response of 57.7% across all extenders was at optimum final seminal temperature and thaw rate 37 degrees C and 18 degrees C/s. Bath temperature and bath time determine optimum thaw rate and final temperature of semen packaged in French straws and thus maximize seminal quality.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0022-0302
pubmed:author
pubmed:issnType
Print
pubmed:volume
67
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1806-12
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Relationship between final temperature, thaw rate, and quality of bovine semen.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't