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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1984-10-25
|
| pubmed:abstractText |
Highly purified and well-characterized samples of precursors and derivatives of bacterial lipopolysaccharides (LPS) were used to study the relationship between the chemical structure of lipid A and its toxicity. These samples included lipids X and Y (monosaccharide precursors of lipid A) from Escherchia coli MN7; incomplete lipid A (a disaccharide precursor of lipid A) from Salmonella typhimurium i50; and monophosphoryl lipid A, TLC-3 (a derivative of LPS), from S. typhimurium G30/C21. In addition, a diphosphoryl lipid A, TLC-3, was prepared from LPS of S. typhimurium G30/C21 and characterized by positive fast atom bombardment mass spectrometry. The diphosphoryl lipid A, TLC-3 fraction, was determined to be very toxic by the chick embryo lethal-dose test (CELD50 = 0.0064 microgram). Lipids X and Y, incomplete lipid A, and monophosphoryl lipid A (TLC-3), were all nontoxic (CELD50 greater than 10 micrograms). These results suggest a multiple structural requirement for toxicity of lipid A. Toxic lipid A must contain all of the following components: a glucosamine disaccharide, a sugar-1-phosphate, and normal fatty acid(s).
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0162-0886
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
6
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
439-43
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading | |
| pubmed:articleTitle |
Separation and characterization of toxic and nontoxic forms of lipid A.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
|