Linked Life Data

6470627

Source:http://linkedlifedata.com/resource/pubmed/id/6470627

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1984-9-25
pubmed:abstractText
A rapid and simple procedure is described for the determination of 4-aminobutyric acid in human CSF. The o-phthaldialdehyde derivative of 4-aminobutyric acid is analysed with a slightly modified commercially available Amino Acid Analyser. We investigated the sample preparation with special emphasis on the sulphosalicylic acid-induced hydrolysis of 4-aminobutyric acid containing compounds in human liquor. Our experiments demonstrate that the amount of estimated 4-aminobutyric acid depends considerably on the sulphosalicylic acid concentration used for protein precipitation. Application of ultrafiltration instead of sulphosalicylic acid precipitation resulted in markedly decreased 4-aminobutyric acid values. By first using ultrafiltration and by minimizing the time between lumbar puncture and analysis, it was shown that protein precipitation with a concentration of sulphosalicylic acid as low as 5 g/l gives 4-aminobutyric acid values in CSF that are essentially correct.
pubmed:language
ger
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0340-076X
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
379-84
pubmed:dateRevised
2007-7-24
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
[Quantitative fluorometric determination of 4-aminobutyric acid in cerebrospinal fluid using an amino acid analyzer].
pubmed:publicationType
Journal Article, English Abstract