| pubmed-article:6469946 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6469946 | lifeskim:mentions | umls-concept:C1135183 | lld:lifeskim |
| pubmed-article:6469946 | lifeskim:mentions | umls-concept:C0021853 | lld:lifeskim |
| pubmed-article:6469946 | lifeskim:mentions | umls-concept:C0450429 | lld:lifeskim |
| pubmed-article:6469946 | lifeskim:mentions | umls-concept:C0006732 | lld:lifeskim |
| pubmed-article:6469946 | lifeskim:mentions | umls-concept:C0039541 | lld:lifeskim |
| pubmed-article:6469946 | lifeskim:mentions | umls-concept:C1516144 | lld:lifeskim |
| pubmed-article:6469946 | lifeskim:mentions | umls-concept:C0597526 | lld:lifeskim |
| pubmed-article:6469946 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:6469946 | pubmed:dateCreated | 1984-10-12 | lld:pubmed |
| pubmed-article:6469946 | pubmed:abstractText | Quantitative analyses were carried out on Tb3+ binding to porcine intestinal calcium-binding protein (CaBP). Tb3+ (emission at 547 nm) and intrinsic tyrosine (emission at 303 nm) fluorescences upon excitation at 260 nm increase almost in parallel with increasing Tb3+ concentration up to a molar ratio of 2 against the protein in the CaBP solution. The pH dependence profile of Tb3+ fluorescence of the Tb3+-CaBP complex suggests that some free carboxylate groups are involved in the binding, as also suggested for Ca2+ binding. The results of fluorometric titration of Tb3+ and intrinsic tyrosine fluorescences of the CaBP complex with Tb3+ or Ca2+ led us to conclude that Tb3+ and Ca2+ have two common binding sites for each CaBP molecule. An equilibrium dialysis experiment showed that the dissociation constants of the two Tb3+-binding sites are 0.29 and 3.51 microM. Tb3+ strongly inhibits 45Ca binding to one of the two Ca2+-binding sites in the CaBP. All of these and previous results indicate that each Tb3+ ion can bind to either of two high-affinity Ca2+-binding sites in porcine intestinal CaBP with an affinity different from that for Ca2+ ion. We discuss the localization of the Ca2+- and Tb3+-binding sites in the CaBP. | lld:pubmed |
| pubmed-article:6469946 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6469946 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6469946 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:6469946 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6469946 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6469946 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6469946 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6469946 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:6469946 | pubmed:issn | 0021-924X | lld:pubmed |
| pubmed-article:6469946 | pubmed:author | pubmed-author:ChibaKK | lld:pubmed |
| pubmed-article:6469946 | pubmed:author | pubmed-author:MohriTT | lld:pubmed |
| pubmed-article:6469946 | pubmed:author | pubmed-author:OhyashikiTT | lld:pubmed |
| pubmed-article:6469946 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6469946 | pubmed:volume | 95 | lld:pubmed |
| pubmed-article:6469946 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6469946 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6469946 | pubmed:pagination | 1767-74 | lld:pubmed |
| pubmed-article:6469946 | pubmed:dateRevised | 2007-12-19 | lld:pubmed |
| pubmed-article:6469946 | pubmed:meshHeading | pubmed-meshheading:6469946-... | lld:pubmed |
| pubmed-article:6469946 | pubmed:meshHeading | pubmed-meshheading:6469946-... | lld:pubmed |
| pubmed-article:6469946 | pubmed:meshHeading | pubmed-meshheading:6469946-... | lld:pubmed |
| pubmed-article:6469946 | pubmed:meshHeading | pubmed-meshheading:6469946-... | lld:pubmed |
| pubmed-article:6469946 | pubmed:meshHeading | pubmed-meshheading:6469946-... | lld:pubmed |
| pubmed-article:6469946 | pubmed:meshHeading | pubmed-meshheading:6469946-... | lld:pubmed |
| pubmed-article:6469946 | pubmed:meshHeading | pubmed-meshheading:6469946-... | lld:pubmed |
| pubmed-article:6469946 | pubmed:meshHeading | pubmed-meshheading:6469946-... | lld:pubmed |
| pubmed-article:6469946 | pubmed:meshHeading | pubmed-meshheading:6469946-... | lld:pubmed |
| pubmed-article:6469946 | pubmed:year | 1984 | lld:pubmed |
| pubmed-article:6469946 | pubmed:articleTitle | Stoichiometry and location of terbium and calcium bindings to porcine intestinal calcium-binding protein. | lld:pubmed |
| pubmed-article:6469946 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6469946 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:6469946 | lld:pubmed |
