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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
1984-10-12
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pubmed:abstractText |
Quantitative analyses were carried out on Tb3+ binding to porcine intestinal calcium-binding protein (CaBP). Tb3+ (emission at 547 nm) and intrinsic tyrosine (emission at 303 nm) fluorescences upon excitation at 260 nm increase almost in parallel with increasing Tb3+ concentration up to a molar ratio of 2 against the protein in the CaBP solution. The pH dependence profile of Tb3+ fluorescence of the Tb3+-CaBP complex suggests that some free carboxylate groups are involved in the binding, as also suggested for Ca2+ binding. The results of fluorometric titration of Tb3+ and intrinsic tyrosine fluorescences of the CaBP complex with Tb3+ or Ca2+ led us to conclude that Tb3+ and Ca2+ have two common binding sites for each CaBP molecule. An equilibrium dialysis experiment showed that the dissociation constants of the two Tb3+-binding sites are 0.29 and 3.51 microM. Tb3+ strongly inhibits 45Ca binding to one of the two Ca2+-binding sites in the CaBP. All of these and previous results indicate that each Tb3+ ion can bind to either of two high-affinity Ca2+-binding sites in porcine intestinal CaBP with an affinity different from that for Ca2+ ion. We discuss the localization of the Ca2+- and Tb3+-binding sites in the CaBP.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-924X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
95
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1767-74
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pubmed:dateRevised |
2007-12-19
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pubmed:meshHeading |
pubmed-meshheading:6469946-Animals,
pubmed-meshheading:6469946-Calcium,
pubmed-meshheading:6469946-Calcium-Binding Proteins,
pubmed-meshheading:6469946-Intestine, Small,
pubmed-meshheading:6469946-Kinetics,
pubmed-meshheading:6469946-Protein Binding,
pubmed-meshheading:6469946-Spectrometry, Fluorescence,
pubmed-meshheading:6469946-Swine,
pubmed-meshheading:6469946-Terbium
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pubmed:year |
1984
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pubmed:articleTitle |
Stoichiometry and location of terbium and calcium bindings to porcine intestinal calcium-binding protein.
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pubmed:publicationType |
Journal Article
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