Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1984-9-17
pubmed:abstractText
Anionic glutathione S-transferases were purified from human lung and placenta. Chemical and immunochemical characterization, including polyacrylamide-gel electrophoresis, gave strong evidence that the anionic lung and placental enzymes are chemically similar, if not identical, proteins. The electrophoretic mobilities of both proteins were identical in conventional alkaline gels as well as in gels containing sodium dodecyl sulphate. Gel filtration of the intact active enzyme established an Mr value of 45000; however, with sodium dodecyl sulphate/polyacrylamide-gel electrophoresis under dissociating conditions a subunit Mr of 22500 was obtained. Amino acid sequence analysis of the N-terminal region of the placental enzyme revealed a single polypeptide sequence identical with that of lung. Results obtained from immunoelectrophoresis, immunotitration, double immunodiffusion and rocket immunoelectrophoresis also indicated the anionic lung and placental enzymes to be closely similar. The chemical similarity of these two proteins was further supported by protein compositional analysis and fragment analysis after chemical hydrolysis. Immunochemical comparison of the anionic lung and placental enzymes with human liver glutathione S-transferases revealed cross-reactivity with the anionic omega enzyme, but no cross-reactivity was detectable with the cationic enzymes. Comparison of the N-terminal region of the human anionic enzyme with reported sequences of rat liver glutathione S-transferases gave strong evidence of chemical similarity, indicating that these enzymes are evolutionarily related. However, computer analysis of the 30-residue N-terminal sequence did not show any significant chemical similarity to any other reported protein sequence, pointing to the fact that the glutathione S-transferases represent a unique class of proteins.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-1262, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-13041735, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-13578996, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-14240539, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-165746, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-16748905, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-28085, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-354496, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-4175439, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-4368334, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-4436300, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-474272, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-4892500, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-532501, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-5398264, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-5959431, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-6120941, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-6187241, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-670218, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-6708590, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-686359, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-6885819, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-7295737, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-7327268, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-7339869, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-7406897, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-7470087, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-7470088, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-903363, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-942051, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-977564, http://linkedlifedata.com/resource/pubmed/commentcorrection/6466318-999809
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
221
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
33-41
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Human glutathione S-transferases. Characterization of the anionic forms from lung and placenta.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.