Linked Life Data

6463585

Source:http://linkedlifedata.com/resource/pubmed/id/6463585

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1984-9-5
pubmed:abstractText
Iron metabolism during inflammation was studied in normal and stimulated peritoneal mouse macrophages. A model was developed for detection of ferritin synthesis in these cells, and inflammatory stimulation was produced by i.p. injection of thioglycollate broth or i.m. injection of turpentine. The cells were adhered to culture discs and incubated at 37 degrees C with 59Fe-transferrin and 3H-leucine, and after washing the cell lysate was chromatographed. In the lysate from stimulated macrophages iron and tritium activity was found in a joint peak, and both were precipitated by a specific anti-mouse ferritin antibody. No significant peaks of radioactivity were found in lysates from normal cells. This showed that inflammatory RES cells have an increased ferritin synthesis. The uptake of 59Fe was investigated in a separate study and the stimulated macrophages were found to have a much higher iron uptake than normal macrophages.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0036-553X
pubmed:author
pubmed:issnType
Print
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
43-8
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Increased ferritin synthesis and iron uptake in inflammatory mouse macrophages.
pubmed:publicationType
Journal Article