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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1982-4-22
|
| pubmed:abstractText |
The interactions of mutant alleles that individually confer radiation sensitivity in Neurospora crassa are being studied with regard to their effects on radiation-induced inactivation and forward-mutation induction at the ad-3 loci. This paper reports attempts to construct 3 double-mutant strains containing the following pair-wise combinations of repair-deficient mutants: upr-1,uvs-2; uvs-2,uvs-6; and uvs-3,uvs-6. The double-mutant strain with the 2 excision-repair-deficient mutants upr-1 and uvs-2 shows increased sensitivity to X-ray-induced mutagenesis and inactivation, relative to that shown by either of the parental single-mutant strains. This double mutant is no more sensitive than the parental single-mutant strains to either UV mutagenesis or inactivation. The combination of the uvs-2 and uvs-6 double-mutant strain is considerably more sensitive to both UV and X-ray inactivation than either the uvs-2 or uvs-6 strain, but it shows no greater sensitivity than the parental strains to ad-3 mutation induction by either agent. The combination of the uvs-3 and uvs-6 alleles is inviable. Tetrad analysis and microscopical examination of ascospores shows that ascospores of presumptive genotype uvs-3, uvs-6 do not grow beyond the formation of a few hyphal threads. The lethal and mutagenic effects of UV and X-irradiation in these double-mutant strains are interpreted in terms of the repair systems in Neurospora and other microorganisms.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0027-5107
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
84
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
49-71
|
| pubmed:dateRevised |
2000-12-18
|
| pubmed:meshHeading |
pubmed-meshheading:6460180-DNA Repair,
pubmed-meshheading:6460180-Light,
pubmed-meshheading:6460180-Mutation,
pubmed-meshheading:6460180-Neurospora,
pubmed-meshheading:6460180-Neurospora crassa,
pubmed-meshheading:6460180-Phenotype,
pubmed-meshheading:6460180-Radiation Tolerance,
pubmed-meshheading:6460180-Ultraviolet Rays,
pubmed-meshheading:6460180-X-Rays
|
| pubmed:year |
1981
|
| pubmed:articleTitle |
Mutagenesis at the ad-3A and ad-3B loci in haploid UV-sensitive strains of Neurospora crassa. V. Comparison of dose--response curves of single- and double-mutant strains with wild-type.
|
| pubmed:publicationType |
Journal Article
|